mProX™ Human DYRK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1106	Magic™ Human DYRK2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DYRK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Intellectual Developmental Disorder, Autosomal Dominant 7. Among its related pathways are Gene expression (Transcription) and Regulation of TP53 Activity

Gene ID

8445

UniProt ID

Q92630

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The DYRK2 gene in humans codes for the enzyme dual-specificity tyrosine-phosphorylation-regulated kinase 2, or more specifically, dual-specificity kinase. As a member of a family of protein kinases, DYRK2 is likely involved in the growth and development of cells. The ability of the family to autophosphorylate on tyrosine residues and the structural similarities of their kinase domains characterize them. In vitro tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B have been demonstrated by DYRK2. DYRK2 has been found to have two isoforms. There is no 5' terminal insert in isoform 1, which is the major isoform. The customized DYRK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Nancy

The DYRK2 cell line worked as advertised and was a cost-effective alternative in our studies. We obtained consistent results, and the cell line showed good stability over extended periods. Mar 07 2020

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chat Jessica

The DYRK2 cell line worked flawlessly, and the detailed protocols provided by the company made the whole process much easier. Nov 07 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 NOTCH1 protein levels are modulated by DYRK2.

After transfecting HEK-293T cells with the designated plasmids, the cells were lysed 48 hours later. Immunoblotting was used to assess the expression of proteins.

Ref: Morrugares, Rosario, et al. "Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2." Cellular and Molecular Life Sciences 77 (2020): 2621-2639.

Pubmed: 31605148

DOI: 10.1007/s00018-019-03309-9

Research Highlights

From yeast to mammals, dual-specificity tyrosine-regulated kinases (DYRKs) have been conserved throughout evolution. A growing body of research has demonstrated the significance of DYRKs in survival and cell cycle regulation.
Yoshida, Saishu, and Kiyotsugu Yoshida. "Multiple functions of DYRK2 in cancer and tissue development." FEBS letters 593.21 (2019): 2953-2965.
Pubmed: 31505048 DOI: 10.1002/1873-3468.13601

These findings demonstrated that DYRK2 expression in HCC tumor tissues was downregulated, which may have encouraged the growth of HCC cells. Furthermore, in HCC, decreased DYRK2 expression was linked to a poor prognosis and oxaliplatin resistance.
Zhang, Xiubing, et al. "Downregulated DYRK2 expression is associated with poor prognosis and Oxaliplatin resistance in hepatocellular carcinoma." Pathology-Research and Practice 212.3 (2016): 162-170.
Pubmed: 26804244 DOI: 10.1016/j.prp.2016.01.002