mProX™ Human DYRK1B Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1105	Magic™ Human DYRK1B in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DYRK1B

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

H184B5F5/M10; MDA-MB-231; MDA-MB-468; HCC1937; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Abdominal Obesity-Metabolic Syndrome 3 and Intellectual Developmental Disorder, Autosomal Dominant 7. Among its related pathways are Neuroscience and Glucose / Energy Metabolism

Gene ID

9149

UniProt ID

Q9Y463

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Tyrosine phosphorylation-regulated kinase with dual specificity 1B is an enzyme that the DYRK1B gene in humans encodes. A protein kinase belonging to the DYRK family is called DYRK1B. Muscle and the testis are the primary locations of DYRK1B, which has a bipartite nuclear localization signal. It has been suggested that the protein regulates nuclear activities. There are three different variants of DYRK1B that differ in having two alternatively spliced exons in the catalytic domain. The customized DYRK1B stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Carol

I've been using the DYRK1B cell line for my studies, and it has been an excellent choice. Dec 13 2020

chat Verified Customer

chat Donna

The DYRK1B cell line showed good performance in our hands. It was reliable for most experiments, and the customer support team was helpful in troubleshooting. Aug 20 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 High throughput screening with the kinome siRNA library for kinases essential for cell viability of TNBC cells.

According to these findings, DYRK1B exhibited the greatest selectivity for cell viability between normal and TNBC cells when compared to the varying outcomes of other candidate gene knockdowns. Three distinct siRNAs were used to effectively silence DYRK1B in order to remove the off-target effects of siRNA.

Ref: Chang, Chia-Che, et al. "Kinome-wide siRNA screening identifies DYRK1B as a potential therapeutic target for triple-negative breast cancer cells." Cancers 13.22 (2021): 5779.

Pubmed: 34830933

DOI: 10.3390/cancers13225779

Research Highlights

When combined, the study's results offer fresh perspectives on the hitherto unknown function of DYRK1B in mitochondrial bioenergetics and the development of ventricular hypertrophy and heart failure. As a result, these discoveries might provide patients with heart failure new treatment alternatives.
Zhuang, Lingfang, et al. "DYRK1B-STAT3 drives cardiac hypertrophy and heart failure by impairing mitochondrial bioenergetics." Circulation 145.11 (2022): 829-846.
Pubmed: 35235343 DOI: 10.1161/CIRCULATIONAHA.121.055727

These results identified Dyrk1b as a therapeutic target for NASH and insulin resistance in the liver and shed light on the mechanisms underlying hepatic lipogenesis and hepatic insulin resistance caused by Dyrk1b.
Bhat, Neha, et al. "Dyrk1b promotes hepatic lipogenesis by bypassing canonical insulin signaling and directly activating mTORC2 in mice." The Journal of Clinical Investigation 132.3 (2022).
Pubmed: 34855620 DOI: 10.1172/JCI153724