mProX™ Human DMPK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1102	Magic™ Human DMPK1(DMPK) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DMPK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

SAOS-2; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Myotonic Dystrophy 1 and Congenital-Onset Steinert Myotonic Dystrophy. Among its related pathways are Cardiac conduction and G-protein signaling RAC1 in cellular process

Gene ID

1760

UniProt ID

Q09013

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Instructions for producing a protein known as myotonic dystrophy protein kinase are found in the DMPK gene. It seems that this protein is crucial for heart, brain, and muscle cells. The protein might have a role in cell-to-cell communication. Via interactions with other proteins, it also seems to control the synthesis and operation of key structures within muscle cells. A set of three repeating DNA building blocks can be found in one area of the DMPK gene. This sequence is referred to as a triplet or trinucleotide repeat and is written as CTG. The majority of persons have between five and thirty-four CTG repeats in this gene. The customized DMPK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Amy

The DMPK cell line was easy to use, and the customer service team was responsive and helpful. Highly recommended! Jun 05 2022

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chat Robert

The technical support team was knowledgeable and provided prompt assistance whenever we had questions. I am extremely satisfied with this DMPK cell line and the overall experience. Feb 27 2022

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FAQ

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Published Data

Fig.1 Human DMPK-A associates with the mitochondria and its kinase activity prevents ROS-induced cell death.

Expression of hDMPK-A in human osteosarcoma SAOS-2 cells that have been transfected stably with plasmids either a wild-type or a fake construct The KD or hDMPK-A hDMPK-A variation. The western immunoblot's loading control was GAPDH.

Ref: Pantic, B., et al. "Myotonic dystrophy protein kinase (DMPK) prevents ROS-induced cell death by assembling a hexokinase II-Src complex on the mitochondrial surface." Cell death & disease 4.10 (2013): e858-e858.

Pubmed: 24136222

DOI: 10.1038/cddis.2013.385

Research Highlights

A newer technique is targeted protein degraders. Their properties are in the 'beyond rule of 5' space, which is distinct from the standard small-molecule property space. As a result, in contrast to conventional small molecules, drug development initiatives aimed at creating orally accessible degraders are anticipated to encounter challenging drug metabolism and pharmacokinetics (DMPK) issues.
Cantrill, Carina, et al. "Fundamental aspects of DMPK optimization of targeted protein degraders." Drug Discovery Today 25.6 (2020): 969-982.
Pubmed: 32298797 DOI: 10.1016/j.drudis.2020.03.012

A clear workflow for DMPK tasks was created, starting with early lead discovery and ending with the choice of a potential medication. This led to a chemical series optimization that was both economical and successful, and it made it easier to make wise decisions as the project moved forward.
Sohlenius-Sternbeck, Anna-Karin, et al. "Optimizing DMPK properties: experiences from a Big Pharma DMPK department." Current Drug Metabolism 17.3 (2016): 253-270.
Pubmed: 26651977 DOI: 10.2174/1389200217666151210125637