mProX™ Human DAPK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1097	Magic™ Human DAPK1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DAPK1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCT116; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Pancreatic Ductal Adenocarcinoma and Cervical Squamous Cell Carcinoma. Among its related pathways are Programmed Cell Death and Dimerization of procaspase-8

Gene ID

1612

UniProt ID

P53355

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human DAPK1 gene encodes the enzyme death-associated protein kinase 1. A positive modulator of gamma-interferon-induced programmed cell death is death-associated protein kinase 1. A 160-kD calmodulin-dependent serine-threonine kinase with two putative P-loop consensus sites and eight ankyrin repeats is encoded by the DAPK1 gene. It is a potential tumor suppressor. The expression of the DAPK1 gene in melanocytic cells may be controlled by MITF. When DAPK1 is depleted from patients with p53-mutant tumors, it inhibits the growth of tumor cells and their count in cellular and animal models of triple receptor-negative breast cancer. The customized DAPK1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The convenience of the DAPK1 cell line has significantly streamlined my workflow. Mar 15 2022

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The DAPK1 cell line is of superior quality, and I am confident in its reliability. Nov 20 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Validation of CRISPR/Cas9-mediated DAPK1 knockout in HCT116 colorectal cancer cells.

Using Western blot, the basal level of DAPK1 protein in the HCT116 cell line was ascertained. In contrast to DLD1 and HT29, which exhibit elevated levels of DAPK1, as well as SW480, SW620, and SW837, where no detectable DAPK1 levels were found, HCT116 exhibits low to moderate amounts of DAPK1.

Ref: Steinmann, Sara, et al. "DAPK1 loss triggers tumor invasion in colorectal tumor cells." Cell death & disease 10.12 (2019): 895.

Pubmed: 31772156

DOI: 10.1038/s41419-019-2122-z

Research Highlights

The Ca2+/calmodulin (CaM)-dependent serine/threonine protein kinase known as death-associated protein kinase 1 (DAPK1) is involved in a number of apoptotic processes, including tumor suppression and neuronal cell death.
Wang, Shan, et al. "DAPK1 signaling pathways in stroke: from mechanisms to therapies." Molecular neurobiology 54.6 (2017): 4716-4722.
Pubmed: 27447806 DOI: 10.1007/s12035-016-0008-y

Thorough research will not only shed light on DAPK1's functions, but it may also lead to the development of new treatments that target the autophagy pathway in cancer in an effort to improve treatment outcomes.
Movahhed, Parvaneh, et al. "The impact of DAPK1 and mTORC1 signaling association on autophagy in cancer." Molecular Biology Reports 49.6 (2022): 4959-4964.
Pubmed: 35083613 DOI: 10.1007/s11033-022-07154-1