mProX™ Human CSNK1G2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1084	Magic™ Human CK1γ2(CSNK1G2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CSNK1G2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Lissencephaly 8 and Familial Febrile Seizures. Among its related pathways are Signaling by WNT and Sphingolipid metabolism

Gene ID

1455

UniProt ID

P78368

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The most susceptible target to TAM was the serine/threonine protein kinase casein kinase 1 gamma 2 (CSNK1G2), which exhibited considerable cytotoxicity in estrogen receptor-positive (ER+) breast cancer cells but very mild damage in ER- cells. In addition, CSNK1G2 knockdown significantly reduced tumor sphere formation and the expression of genes associated with breast stem cells, such as CD44/CD2. CSNK1G2 consistently changed the regulation of the estrogen-responsive element (ERE) of estrogen-responsive genes including CTSD and GREB1 and phosphorylated ERα activity at serine (Ser)167. ERα knockdown, however, virtually totally prevented CSNK1G2-induced TAM sensitivity. The customized CSNK1G2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jennifer

This CSNK1G2 cell line is essential for our study. Applications of this cell line can be developed through more innovation and customisation of the protocol. Mar 27 2020

chat Verified Customer

chat Jessica

I choose the CSNK1G2 cell line with luminescent signals. This makes my testing steps less complicated. Sep 21 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Human CSNK1G2 interacts with DUOXA2 and promotes ROS levels.

Immunofluorescent staining demonstrating the colocalization of FLAG::CSNK1G2 (red) with DUOXA2::HA (green) on the membrane and subcellular level in HEK293T cells.

Ref: Hu, Yiman, et al. "Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex." PLoS Genetics 19.4 (2023): e1010740.

Pubmed: 37099597

DOI: 10.1371/journal.pgen.1010740

Research Highlights

These findings imply that CSNK1G2 regulates PI3K/AKT/mTOR/S6K and ERK signaling differently, which is important in sensitizing TAM toxicity in ER+ and ER- breast cancer cells.
Nguyen Hoang, Anh Thu, Kwang-Lae Hoe, and Sook-Jeong Lee. "CSNK1G2 differently sensitizes tamoxifen-induced decrease in PI3K/AKT/mTOR/S6K and ERK signaling according to the estrogen receptor existence in breast cancer cells." Plos one 16.4 (2021): e0246264.
Pubmed: 33861751 DOI: 10.1371/journal.pone.0246264

CSNK1G2-AS1 may be crucial for the development and spread of TGCTs via the AKT- and EMT-mediated signaling pathways.
Li, Ruixue, et al. "CSNK1G2-AS1 promotes metastasis, colony formation and serves as a biomarker in testicular germ cell tumor cells." Journal of Cancer 14.15 (2023): 2771.
Pubmed: 37781070 DOI: 10.7150/jca.85640