mProX™ Human CSNK1E Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1087	Magic™ Human CK1ε(CSNK1E) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CSNK1E

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MCF7; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Developmental And Epileptic Encephalopathy 1 and West Syndrome. Among its related pathways are Loss of Nlp from mitotic centrosomes and ncRNAs involved in Wnt signaling in hepatocellular carcinoma

Gene ID

1454

UniProt ID

P49674

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Kinase of casein The CSNK1E gene in humans codes for the enzyme epsilon isoform. This gene codes for a serine/threonine protein kinase, which has been linked to the regulation of nuclear and cytoplasmic functions, such as DNA replication and repair. The encoded protein can phosphorylate many different proteins, including itself. It is present in the cytoplasm as a monomer. It has been demonstrated that the Period family of circadian rhythm proteins is phosphorylated by this protein. Drosophila melanogaster has a homolog of this human protein. This protein, also referred to as doubletime, is involved in the phosphorylation of other proteins that are involved in circadian rhythms. For this gene, two transcript variants that encode the same protein have been discovered. The customized CSNK1E stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The CSNK1E cell line has proven valuable in various experimental setups, and its compatibility with different techniques makes it a versatile asset for our investigations. May 11 2023

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I believe this is a simple, sensitive and effective CSNK1E cell line. Jun 02 2023

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FAQ

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Published Data

Fig.1 Loss of CSNK1E function abrogates Wnt/β-catenin signaling.

A β-catenin expression construct, pRL-SV40, the specified shRNA construct, and the β-catenin-responsive pTOPFLASH reporter constructs were cotransfected into 293T cells. Immunoblot study of MCF7 cells 72 hours after transduction with shRNA against CSNK1E, showing levels of nuclear (right) and cytoplasmic (left) β-catenin.

Ref: Kim, So Young, et al. "CK1ε is required for breast cancers dependent on β-catenin activity." PloS one 5.2 (2010): e8979.

Pubmed: 20126544

DOI: 10.1371/journal.pone.0008979

Research Highlights

Finding circadian genes that are differently expressed in KIRC and evaluating their significance in the development of KIRC were the objectives of this investigation. There were 553 differentially expressed rhythm genes (DERGs) in KIRC; of them, 253 were down-regulated and 300 were up-regulated.
Li, Shujing, et al. "Effects and prognostic values of circadian genes CSNK1E/GNA11/KLF9/THRAP3 in kidney renal clear cell carcinoma via a comprehensive analysis." Bioengineering 9.7 (2022): 306.
Pubmed: 35877357 DOI: 10.3390/bioengineering9070306

In this Korean population, CSNK1E SNP rs2075984 appeared to be crucial in the onset of bipolar disorder. The phase preference as determined by the CS does not appear to be related to this connection. Larger sample sizes and additional SNPs are required for further research on CSNK1E.
Lee, Kyu Young, et al. "Genetic association study of CSNK1E gene in bipolar disorder and circadian characteristics." Nordic Journal of Psychiatry 72.8 (2018): 599-604.
Pubmed: 30445897 DOI: 10.1080/08039488.2018.1509125