mProX™ Human CLK4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1093	Magic™ Human CLK4 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CLK4

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

KYSE510; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Reproductive Research

Related Diseases

Fanconi Anemia, Complementation Group A. Among its related pathways are Processing of Capped Intron-Containing Pre-mRNA

Gene ID

57396

UniProt ID

Q9HAZ1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The CDC2-like protein kinase (CLK) family includes the CLK4 protein. The serine- and arginine-rich (SR) proteins are known to be crucial for the development of spliceosomes, and as such, they may be regulated by this protein kinase through interaction and phosphorylation. Research conducted on the Israeli sand rat Psammomys obesus revealed that this kinase may interact with and be regulated by the highly conserved ubiquitin-like protein known as ubiquitin-like 5 (UBL5/BEACON). Numerous transcript variants with alternative splicing have been detected; nevertheless, their complete natures remain unknown at this time. The customized CLK4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Charles

With the CLK4 cell line, I can now complete my experiments in a fraction of the time. Aug 09 2021

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chat Laura

The CLK4 cell line is a cost-effective solution that has enhanced the efficiency of my lab work significantly. Aug 25 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CDC-like kinase 4 (CLK4) phosphorylates microphthalmia-associated transcription factor (MITF) at Y360.

WT-Flag-CLK4 or the kinase-deficient mutant Flag-CLK4-K189R were transfected into KYSE510 cell lines. Next, anti-phosphor-ser/thr and anti-tyrosine 4G10 antibodies were used to investigate the immunoprecipitated MITF, respectively.

Ref: Shen, Yaxing, et al. "Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy." Clinical and Translational Medicine 12.1 (2022): e719.

Pubmed: 35092699

DOI: 10.1002/ctm2.719

Research Highlights

These findings imply that CLK4 is essential for the invasion and growth of MES-TNBC, particularly during TGF-β-induced processes. Furthermore, CLK4 is described in this work as a unique therapeutic target in breast cancer.
Kang, Eunji, et al. "Targeting CLK4 inhibits the metastasis and progression of breast cancer by inactivating TGF-β pathway." Cancer Gene Therapy 29.8-9 (2022): 1168-1180.
Pubmed: 35046528 DOI: 10.1038/s41417-021-00419-0

These findings offer potential therapeutic targets for the treatment of ESCC by highlighting the possible function of CLK4 in altering redox state and nucleotide metabolism.
Shen, Yaxing, et al. "Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy." Clinical and Translational Medicine 12.1 (2022): e719.
Pubmed: 35092699 DOI: 10.1002/ctm2.719