mProX™ Human CLK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1090	Magic™ Human CLK1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CLK1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

BxPC-3; PANC-1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Related Diseases

Among its related pathways are Processing of Capped Intron-Containing Pre-mRNA

Gene ID

1195

UniProt ID

P49759

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Two-species protein kinase The CLK1 gene in humans codes for the enzyme CLK1. A dual specificity protein kinase belonging to the CDC2-like (or LAMMER) family is encoded by this gene. Serine/arginine-rich proteins involved in pre-mRNA processing are phosphorylated by the encoded protein in the cell nucleus, releasing them into the nucleoplasm. The concentration of transacting factors, such as serine/arginine-rich proteins, may control the selection of splice sites during pre-mRNA processing. As a result, splice site selection may be indirectly regulated by the encoded protein. The customized CLK1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Cynthia

Despite its competitive price, the CLK1 cell line exhibited excellent binding affinity and sensitivity comparable to more expensive alternatives. Sep 17 2020

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chat Robert

The CLK1 cell line is a fantastic solution for researchers looking to save money without compromising quality. Oct 14 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CLK1 promoted the migration and invasion ability of pancreatic cells in vitro and in vivo.

Stable transfection of BxPC-3 and PANC-1 cells was used in transwell experiments. Results are presented with quantification and representative photos. Stably transfected BxPC-3 and PANC-1 cells were used in wound-healing experiments. There are shown representative photos and a quantification of wound closure.

Ref: Chen, Shi, et al. "CLK1/SRSF5 pathway induces aberrant exon skipping of METTL14 and Cyclin L2 and promotes growth and metastasis of pancreatic cancer." Journal of hematology & oncology 14.1 (2021): 1-24.

Pubmed: 33849617

DOI: 10.1186/s13045-021-01072-8

Research Highlights

This work reveals a novel chemical probe for elucidating the role of trypanosomatid parasite protozoa's divergent kinetochores, as well as a novel therapeutic target for these parasitic protozoa.
Saldivia, Manuel, et al. "Targeting the trypanosome kinetochore with CLK1 protein kinase inhibitors." Nature microbiology 5.10 (2020): 1207-1216.
Pubmed: 32661312 DOI: 10.1038/s41564-020-0745-6

By shaping U1-70K for protein-protein multitasking, these experiments show that CLK1 plays a crucial, signal-dependent function in early spliceosomal protein building.
Aubol, Brandon E., et al. "CLK1 reorganizes the splicing factor U1-70K for early spliceosomal protein assembly." Proceedings of the National Academy of Sciences 118.14 (2021): e2018251118.
Pubmed: 33811140 DOI: 10.1073/pnas.2018251118