mProX™ Human CHEK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1081	Magic™ Human CHK2(CHEK2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CHEK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CRC; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Prostate Cancer and Li-Fraumeni Syndrome 2. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Homology Directed Repair

Gene ID

11200

UniProt ID

O96017

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The serine-threonine kinase CHK2 is encoded by the tumor suppressor gene CHEK2 (Checkpoint kinase 2). In response to DNA damage, CHK2 is engaged in DNA repair, cell cycle arrest, or apoptosis. Numerous malignancies have been connected to mutations in the CHEK2 gene. Checkpoint kinase 2 (CHK2) is a tumor suppressor protein that is encoded by the CHEK2 gene. As a regulator of cell division, CHK2 can stop cells from proliferating too quickly or uncontrollably. Additionally, it is known that CHK2 phosphorylates the promyelocytic leukemia protein (PML), which is involved in apoptosis (programmed cell death), as well as the cell-cycle transcription factor E2F1. The customized CHEK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Michelle

I recently utilized the CHEK2 KO cell line from Creative Biolabs. This cell line proved to be an exceptional targeting tool for my research. Sep 26 2021

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chat Jennifer

The CHEK2 KO cell line has been crucial in elucidating the receptor's role in various biological processes. Oct 16 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CRISPR/Cas9-mediated CHEK2 gene inactivation in CRC cell lines.

Western blot analysis verified that CHEK2KO cells had completely lost the ability to produce Chk2. Following CHEK2 KO, there was no effect on cell proliferation. This suggested that the functional characterisation of CHEK2 may be investigated using this CRISPR cell model.

Ref: Xu, Pingping, et al. "Germline mutations in a DNA repair pathway are associated with familial colorectal cancer." JCI insight 6.18 (2021).

Pubmed: 34549727

DOI: 10.1172/jci.insight.148931

Research Highlights

Hereditary cancer risk has been linked to germline mutations in numerous genes that code for proteins mediating DNA repair and DNA damage response (DDR) to double-strand breaks (DDSB).
Stolarova, Lenka, et al. "CHEK2 germline variants in cancer predisposition: stalemate rather than checkmate." Cells 9.12 (2020): 2675.
Pubmed: 33322746 DOI: 10.3390/cells9122675

While CHEK2 PVs were linked to thyroid, kidney, and breast malignancies, they were not linked to colorectal cancer. The common p.I157T, p.S428F, and p.T476M alleles were not linked to nonbreast malignancies and had a less correlation with breast cancer when compared to other CHEK2 PVs. The treatment and genetic counseling provided to people with CHEK2 PVs may benefit from these data.
Bychkovsky, Brittany L., et al. "Differences in Cancer Phenotypes Among Frequent CHEK2 Variants and Implications for Clinical Care-Checking CHEK2." JAMA oncology 8.11 (2022): 1598-1606.
Pubmed: 36136322 DOI: 10.1001/jamaoncol.2022.4071