mProX™ Human CDK6 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1073	Magic™ Human CDK6/CycD1 in Vitro Assay	Human		Kinase Assay
S01YF-1122-KX1074	Magic™ Human CDK6/CycD3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK6

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

COLO320; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Microcephaly 12, Primary, Autosomal Recessive and Primary Autosomal Recessive Microcephaly. Among its related pathways are Aberrant regulation of mitotic G1/S transition in cancer due to RB1 defects and Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6

Gene ID

1021

UniProt ID

Q00534

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The enzyme known as cell division protein kinase 6 (CDK6) is produced by the CDK6 gene. This gene codes for a protein that is a part of the CDK (cyclin-dependent kinase) family, which also contains CDK4. This kinase is a catalytic subunit of the protein kinase complex, which is also made up of the activating subunit cyclin D. The complex is crucial for the G1 phase advancement and G1/S transition of the cell cycle. Mid-G1 phase, when this kinase's activity initially manifests, is regulated by components such as D-type cyclins and CDK inhibitors belonging to the INK4 family. CDK6 is a crucial protein in cancer since it has been demonstrated that this kinase and CDK4 phosphorylate the tumor suppressor Retinoblastoma protein, hence controlling its activity. The customized CDK6 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Elizabeth

This human CDK6 cell line is highly selective. Its performance has been outstanding, demonstrating reliable and consistent binding. Mar 10 2022

chat Verified Customer

chat Linda

This CDK6 cell line can be applied well according to experimental needs. Dec 02 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CDK6 phosphorylates RB for cancer cell growth.

Lentivral vectors expressing CDK4 shRNA sequences (CDK4-20, CDK4-64) and CDK6 shRNA sequences (CDK6-893, CDK6-747) were used to transduce COLO320 cells. The cells were then subjected to western blotting with the antibodies as stated (left of panel).

Ref: Li, Chunsheng, et al. "PD‑0332991 induces G1 arrest of colorectal carcinoma cells through inhibition of the cyclin‑dependent kinase‑6 and retinoblastoma protein axis." Oncology letters 7.5 (2014): 1673-1678.

Pubmed: 24765199

DOI: 10.3892/ol.2014.1957

Research Highlights

Cyclin-dependent kinase 6 (CDK6) is essential for controlling how the cell cycle develops. It has also been demonstrated more recently that CDK6 plays a transcriptional role in tumor angiogenesis.
Tadesse, Solomon, et al. "Targeting CDK6 in cancer: State of the art and new insights." Cell Cycle 14.20 (2015): 3220-3230.
Pubmed: 26315616 DOI: 10.1080/15384101.2015.1084445

Both hematopoietic stem cells and tumor cells use CDK6 directly for transcription. These activities suggest that CDK6 has a role in tissue homeostasis and differentiation that is distinct from CDK4 and partially independent of CDK6 kinase activity.
Tigan, A. S., et al. "CDK6-a review of the past and a glimpse into the future: from cell-cycle control to transcriptional regulation." Oncogene 35.24 (2016): 3083-3091.
Pubmed: 26500059 DOI: 10.1038/onc.2015.407