mProX™ Human CDK19 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1065	Magic™ Human CDK19/CycC in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK19

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Inflammation Research

Related Diseases

Developmental And Epileptic Encephalopathy 87 and Non-Specific Early-Onset Epileptic Encephalopathy. Among its related pathways are PPARA activates gene expression and Metabolism

Gene ID

23097

UniProt ID

Q9BWU1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Although CDK19 is conserved in model organisms that are both vertebrate and invertebrate, defects in CDK19 are not currently linked to any disorders in humans. Larval lethality is caused by loss of Cdk8, the fly homolog of CDK19, and is prevented by expression of the human CDK19 reference cDNA. Conversely, the CDK19 p.Tyr32His and p.Thr196Ala variations found in the afflicted people act as null alleles and are unable to compensate for the loss of Cdk8. Furthermore, semi-lethality is the outcome of Cdk8 knockdown in flies using neuronal RNAi. The few flies that eclose show signs of severe seizures and a shorter life span. Moderate expression of the CDK19 standard cDNA completely suppresses both symptoms, whereas expression of the two variations does not. The customized CDK19 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jennifer

We obtained good data in downstream analysis using this CDK19 cell line. May 07 2023

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chat Brian

A good CDK19 cell line. The company obtains a wide range of membrane protein products which are very reliable. Aug 18 2022

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FAQ

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Published Data

Fig.1 CDK8/19 kinase activity is necessary for intestinal epithelial cell intrinsic growth and differentiation.

Small intestine organoids from mice treated with 4-OHT for 24 hours, one week later, were immunoblotted from VillinCreER T2, VillinCreER T2 /Cdk8 fl/fl, and VillinCreER T2 /Cdk8 fl/fl /Cdk19 -/-mice.

Ref: Dannappel, Marius V., et al. "CDK8 and CDK19 regulate intestinal differentiation and homeostasis via the chromatin remodeling complex SWI/SNF." The Journal of clinical investigation 132.20 (2022).

Pubmed: 36006697

DOI: 10.1172/JCI158593

Research Highlights

Known together as "Mediator Kinases," CDK8 and its paralog, CDK19, are cyclin-dependent kinases that have been identified as important regulators of cellular homeostasis and developmental programming.
Dannappel, Marius Volker, et al. "Molecular and in vivo functions of the CDK8 and CDK19 kinase modules." Frontiers in cell and developmental biology 6 (2019): 171.
Pubmed: 30693281 DOI: 10.3389/fcell.2018.00171

While CDK8 and CDK19 have similar qualitative effects on gene expression at the RNA and protein levels and on protein phosphorylation, analysis of isogenic cell populations expressing CDK8, CDK19, or their kinase-inactive mutants showed that the differences in the effects of CDK8 versus CDK19 knockouts were due to quantitative variations in their expression and activity rather than distinct functions.
Chen, Mengqian, et al. "CDK8 and CDK19: positive regulators of signal-induced transcription and negative regulators of Mediator complex proteins." Nucleic Acids Research 51.14 (2023): 7288-7313.
Pubmed: 37378433 DOI: 10.1093/nar/gkad538