mProX™ Human CDK18 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1064	Magic™ Human CDK18/CycY in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK18

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HeLa; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Gene ID

5129

UniProt ID

Q07002

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Serine-threonine protein kinase PCTAIRE kinase 3/cyclin-dependent kinase 18 (PCTK3/CDK18) is a member of the CDK family. While PCTK3 is expressed in distinct organs and cell types, it is possible that PCTK3 is involved in particular tasks in post-mitotic cells, despite the fact that CDKs are broadly expressed in mammalian cells. For complete activation, the CDK family needs a certain regulatory component and/or post-translational protein modification. Retinoblastoma protein (Rb) is phosphorylated in vitro by activated PCTK3. The downstream signaling pathway of PCTK3 is still unknown, despite the fact that its activation mechanism is gradually becoming more understood. The customized CDK18 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The CDK18 overexpression cell line is an essential tool for anyone studying drug discovery. Apr 16 2023

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The CDK18 KD cell line is very good to use and is of high quality and safety. Dec 15 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CDK18-depleted cells fail to elicit robust replication stress signaling.

Measurement of phosphorylated RPA2 (phospho Thr21) foci in HeLa cells treated with 3 mM HU for 6 hours after transfecting them with either single or control CDK18 siRNAs. Cell populations that were not under stress showed no discernible variations.

Ref: Barone, Giancarlo, et al. "Human CDK18 promotes replication stress signaling and genome stability." Nucleic acids research 44.18 (2016): 8772-8785.

Pubmed: 27382066

DOI: 10.1093/nar/gkw615

Research Highlights

These results identify CDK18 as a novel regulator of genome integrity and show that it has a rate-limiting function in replication stress signaling.
Barone, Giancarlo, et al. "Human CDK18 promotes replication stress signaling and genome stability." Nucleic acids research 44.18 (2016): 8772-8785.
Pubmed: 27382066 DOI: 10.1093/nar/gkw615

These findings imply that there may be varied patient outcomes predicted depending on how differentially CDK18 mRNA and protein levels are controlled inside cancer cells.
Braams, Esmée, and Vincenzo D'Angiolella. "Keeping CDK18 in balance to prevent DNA replication stress in breast cancer." Oncotarget 9.102 (2018): 37610.
Pubmed: 30701016 DOI: 10.18632/oncotarget.26517