mProX™ Human CDK16 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1062	Magic™ Human CDK16/CycY in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK16

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MDA-MB-231; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Microcephaly. Among its related pathways are Activation of cAMP-Dependent PKA and DNA Damage

Gene ID

5127

UniProt ID

Q00536

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Activated in the cell cycle, vesicle trafficking, spindle orientation, skeletal myogenesis, neurite outgrowth, secretory cargo transport, spermatogenesis, glucose transportation, cell apoptosis, growth and proliferation, metastasis, and autophagy, cyclin-dependent kinase 16 (CDK16) is an orphan "cyclin-dependent kinase" (CDK). Human CDK16 is associated with X-linked congenital disorders and is found on chromosome Xp11.3. Mammalian tissues frequently express CDK16, which has the potential to function as an oncoprotein. This PCTAIRE kinase is regulated by binding to the N- and C-terminal domains of CDK16 by Cyclin Y or its ortholog, Cyclin Y-like 1. Malignant melanoma, hepatocellular carcinoma, lung cancer, prostate cancer, and breast cancer are among the cancers for which CDK16 is essential. A promising biomarker for cancer prognosis and diagnosis is CDK16. The customized CDK16 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Joseph

The human CDK16 overexpression cell line is an excellent product that has exceeded my expectations. Dec 04 2022

chat Verified Customer

chat Michelle

The CDK16 cell line is easy to use, and the results obtained have been consistent and reliable. I would definitely recommend it to others. Feb 17 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CDK16 regulates cell proliferation by phosphorylating PRC1 and modulating spindle formation.

Analysis of spindle formation in CDK16-KD and control MDA-MB-231 cells. Antibodies against pericentrin (red), alpha-tubulin (green), and DAPI (blue) were used to immunostain the cells.

Ref: Li, Xiao, et al. "CDK16 promotes the progression and metastasis of triple-negative breast cancer by phosphorylating PRC1." Journal of Experimental & Clinical Cancer Research 41.1 (2022): 149.

Pubmed: 35449080

DOI: 10.1186/s13046-022-02362-w

Research Highlights

Triple-negative breast cancer (TNBC) is known to exhibit elevated expression of CDK16. There is a correlation between worse outcomes for patients with breast cancer and higher CDK16 expression.
Li, Xiao, et al. "CDK16 promotes the progression and metastasis of triple-negative breast cancer by phosphorylating PRC1." Journal of Experimental & Clinical Cancer Research 41.1 (2022): 149.
Pubmed: 35449080 DOI: 10.1186/s13046-022-02362-w

In human NSCLC tumor tissue, CDK16 is markedly overexpressed. It promotes cell proliferation and, in a p27-dependent way, inhibits apoptosis in NSCLC cells, thereby acting as an oncogene. Moreover, CDK16 uses protein degradation and ubiquitination to negatively regulate p27 production.
Wang, Hongtao, et al. "CDK16 overexpressed in non-small cell lung cancer and regulates cancer cell growth and apoptosis via a p27-dependent mechanism." Biomedicine & Pharmacotherapy 103 (2018): 399-405.
Pubmed: 29674275 DOI: 10.1016/j.biopha.2018.04.080