mProX™ Human CDC7 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1057	Magic™ Human CDC7/ASK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDC7

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCC; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Ocular Research

Related Diseases

Glaucoma, Primary Open Angle. Among its related pathways are Activation of the pre-replicative complex and Gene expression (Transcription)

Gene ID

8317

UniProt ID

O00311

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. At the stage of chromosomal DNA replication, the Cdc7 kinase regulates the cell cycle. Since the Cdc7 kinase protein is present in the majority of eukaryotic cells, the gene CDC7 appears to have been conserved throughout the evolution of eukaryotic cells. The protein that this gene codes for is a kinase-active cell division cycle protein that is primarily found in the nucleus. The protein regulates the course of the cell cycle and is necessary for the start of DNA replication. For certain cancers, overexpression of this gene product might be linked to neoplastic transformation. The discovery of additional transcript sizes points to the possibility of alternative splicing. The customized CDC7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Charles

This is a very reliable CDC7 KD cell line with high sensitivity and specificity, suitable for experiments such as immunofluorescence microscopy and flow cytometry. May 14 2023

chat Verified Customer

chat Emily

Recommended for all labs based on my experience of using this CDC7 KO cell line. Jun 28 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Combined CDC7 and ATR-CHK1 inhibition leads to defective cell cycle of HCC cells.

Typical live cell pictures of GFP-Histone 2B-expressing PLC/PRF/5 and SNU449 cells. Time-lapse microscopy was used to track cells treated with XL413 (10 μM), AZD6738 (1.25 μM), MK-8776 (2.5 μM), or the indicated combinations.

Ref: Guo, Yuchen, et al. "Targeting CDC7 potentiates ATR-CHK1 signaling inhibition through induction of DNA replication stress in liver cancer." Genome Medicine 13.1 (2021): 1-15.

Pubmed: 34663432

DOI: 10.1186/s13073-021-00981-0

Research Highlights

Chemotherapeutic medicines that target replication stress (RS), a hallmark of cancer, are commonly utilized to treat a variety of malignancies. The development of next-generation anticancer medicines that induce replication stress (RS) has focused on cell division cycle 7 (CDC7) as a target.
Iwai, Kenichi, et al. "Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor." Science advances 5.5 (2019): eaav3660.
Pubmed: 31131319 DOI: 10.1126/sciadv.aav3660

These data challenge our knowledge of how the cell cycle proceeds by showing that, while CDC7 serves a redundant role in DNA replication, CDK1 physiologically controls two different transitions during the cell division cycle.
Suski, Jan M., et al. "CDC7-independent G1/S transition revealed by targeted protein degradation." Nature 605.7909 (2022): 357-365.
Pubmed: 35508654 DOI: 10.1038/s41586-022-04698-x