mProX™ Human CDC20 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1046	Magic™ Human BUB1/BUB3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDC20

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HeLa; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Mosaic Variegated Aneuploidy Syndrome 1 and Mosaic Variegated Aneuploidy Syndrome. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and EML4 and NUDC in mitotic spindle formation

Gene ID

991

UniProt ID

Q12834

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The CDC20 gene in humans encodes the cell division cycle protein 20 homolog, an important regulator of cell division. To the best of our understanding, its primary role is to trigger the massive 11-13 component complex known as the anaphase promoting complex (APC/C), which is responsible for starting chromatid separation and the anaphase transition.It destroys securin, which eventually leads to the breakdown of cohesin and sister chromatid separation. Additionally, it destroys S and M-phase (S/M) cyclins, inactivating S/M cyclin-dependent kinases (Cdks) and permitting the cell to leave mitosis. At various stages of the cell cycle, CDC20 interacts with numerous other proteins, seemingly serving as a regulatory protein. Two microtubule-dependent activities depend on it: nuclear migration before anaphase, and chromosome separation. The customized CDC20 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Deborah

The CDC20 cell line is easy to work with and consistently provides reliable results. Jun 28 2023

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chat Gary

The human CDC20 cell line was easy to use, saving me valuable time in the lab. Mar 18 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Cdc20 phosphorylation prevents interphase APC/C binding.

After removing endogenous Cdc20 from stable HeLa cell lines expressing the corresponding YFP-tagged Cdc20 proteins, YFP-Cdc20 complexes were purified eight hours after the cells were released from a thymidine block. SDS-PAGE was used to analyze the samples, and LI-COR technology was used to quantify the results of probing for APC1, APC3, and APC7.

Ref: Hein, Jamin B., and Jakob Nilsson. "Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry." Nature communications 7.1 (2016): 10975.

Pubmed: 26960431

DOI: 10.1038/ncomms10975

Research Highlights

The Anaphase Promoting Complex (APC, also known as APC/C) forms two functionally independent but functionally linked E3 ubiquitin ligase sub-complexes, APC(Cdc20) and APC(Cdh1), respectively, that govern the advancement of the cell cycle.
Wang, Lixia, et al. "Targeting Cdc20 as a novel cancer therapeutic strategy." Pharmacology & therapeutics 151 (2015): 141-151.
Pubmed: 25850036 DOI: 10.1016/j.pharmthera.2015.04.002

Important roles for cell-division cycle protein 20 homologue (Cdc20) include chromosomal segregation and mitotic exit. Targeted by the spindle assembly checkpoint (SAC), Cdc20 is also an essential cofactor of the anaphase-promoting complex or cyclosome (APC/C) E3 ubiquitin ligase, which controls APC/C ubiquitin activity on particular substrates in preparation for the proteasome's eventual destruction.
Kapanidou, Maria, Natalie L. Curtis, and Victor M. Bolanos-Garcia. "Cdc20: at the crossroads between chromosome segregation and mitotic exit." Trends in biochemical sciences 42.3 (2017): 193-205.
Pubmed: 28202332 DOI: 10.1016/j.tibs.2016.12.001