mProX™ Human CD40LG Stable Cell Line

Product Information

Target Family

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;Human T cells

Target Classification

CD Cell Lines

Target Research Area

Immunology Research

Related Diseases

Immunodeficiency With Hyper-Igm, Type 1; Cd40 Ligand Deficiency

Gene ID

Human:959

UniProt ID

Human:P29965

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

CD40LG, the ligand for the CD40 receptor, has various applications in different fields. In chronic inflammatory diseases, the soluble CD40 receptor (sCD40) isoform generated by proteolytic cleavage or alternative splicing of CD40 is implicated in the pathogenesis of these diseases. It is also considered a potential biomarker for chronic inflammatory diseases. In people living with HIV, high levels of soluble CD40 ligand (sCD40L) are associated with metabolic syndrome, a condition characterized by endothelial dysfunction. This suggests a potential role for sCD40L in the association between cardiometabolic comorbidity and HIV infection. In patients with complex regional pain syndrome (CRPS), inflammation induces the expression of Œ±(1)-adrenoceptor in peripheral blood mononuclear cells (PBMC). This inflammatory cascade, along with elevated levels of IL-6, may contribute to systemic inflammation and antibody production in CRPS. Additionally, CD40 ligand is investigated as a potential adjuvant for the development of an efficient HIV-1 therapeutic vaccine. The linkage of CD40L to the Nef antigen can induce the Th-1 pathway and activate cytotoxic T lymphocytes. Finally, in Parkinson's disease, abnormal B-lymphocyte immune responses in peripheral blood, including alterations in B-cell subsets and increased production of cytokines, are associated with the development of the disease. Elevated levels of soluble CD40 ligand, along with other cytokines, may serve as potential targets for intervention in Parkinson's disease.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Skyler Brown (Verified Customer)

What is the significance of CD40LG in immune responses? Dec 16 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

CD40LG plays a crucial role in immune responses, particularly in activating and modulating immune cells. Its expression and function are significant in various diseases, including cancer and immunodeficiencies. Dec 16 2022

chat Skyler Garcia (Verified Customer)

How does CD40LG mutation affect health? Apr 10 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

CD40LG mutations can lead to conditions like X-linked hyper IgM syndrome, impacting immune system functioning and increasing susceptibility to infections. Apr 10 2020

Published Data

Fig.1 Overexpressing CD40LG in human T cells.

Primary T cells from normal human donors were transfected with CD40LG cDNA, which had been cloned into the expression vector pEGFP-C1 (pEGFP-C1/CD40LG), while cells transfected with the empty vector were used as controls. The confirmation of transfection efficiency (≥ 40%) was carried out through flow cytometry for the GFP protein. CD40LG overexpression in transfected T cells was subsequently confirmed through the utilization of a fluorochrome-conjugated anti-CD40LG antibody in flow cytometry analysis.

Ref: Zhou, Ying, et al. "T cell CD40LG gene expression and the production of IgG by autologous B cells in systemic lupus erythematosus." Clinical immunology 132.3 (2009): 362-370.

Pubmed: 19520616

DOI: 10.1016/j.clim.2009.05.011

Research Highlights

H Wagner, A. et al. "Potential role of soluble CD40 receptor in chronic inflammatory diseases." Biochemical pharmacology, 2023.
The CD40 receptor and its ligand CD154 have a broad expression in immune-competent cells. The interaction between CD154 and CD40 is crucial for the growth and differentiation of B-cells, as well as immunoglobulin class switching. This co-stimulatory ligand-receptor pair also plays a significant role in the communication between various immune-competent cells involved in innate and adaptive immunity. The CD40-CD154 interaction has a significant involvement in the development of inflammatory and autoimmune diseases. Although both CD40 and CD154 are membrane-bound proteins, their soluble forms can be generated through proteolytic cleavage or alternative splicing. In this review, current knowledge about single nucleotide polymorphisms in the human CD40 gene and compensatory changes in the plasma level of soluble CD40 receptor (sCD40) isoform in related pro-inflammatory diseases will be summarized. Additionally, the regulation patterns of the disintegrin metalloprotease ADAM17 function, which leads to the shedding of transmembrane proteins, such as pro-inflammatory adhesion molecules or CD40, will be discussed. Finally, the potential of sCD40 as a biomarker for chronic inflammatory diseases will also be explored.
H Wagner, A. et al. "Potential role of soluble CD40 receptor in chronic inflammatory diseases." Biochemical pharmacology, 2023.
Pubmed: 37863325 DOI: 10.1016/j.bcp.2023.115858

B Jacobsen, Mads-Holger. et al. "Metabolic syndrome, soluble CD40L, and biomarkers of endothelial dysfunction in people living with HIV." APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 2023.
The aim of this present study was to investigate the relationship between soluble CD40 ligand (sCD40L), a marker of platelet activation, and soluble thrombomodulin and syndecan-1, both markers of endothelial dysfunction, with metabolic syndrome in a large cohort of well-treated people with HIV (PWH). The study included 862 PWH with undetectable viral replication. The authors used uni- and multivariable logistic regression models, adjusted for known confounders, to test their hypotheses. While there was no association found between soluble thrombomodulin and syndecan-1 with metabolic syndrome, high levels of sCD40L were significantly associated with increased risk of metabolic syndrome (adjusted odds ratio 1.54, 95% CI 1.07-2.22). These results suggest a potential role for sCD40L in the well-known link between cardiometabolic comorbidity and HIV infection, given its known association with vascular inflammation and endothelial damage.
B Jacobsen, Mads-Holger. et al. "Metabolic syndrome, soluble CD40L, and biomarkers of endothelial dysfunction in people living with HIV." APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 2023.
Pubmed: 37849049 DOI: 10.1111/apm.13354