mProX™ Human CAMK2G Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1052	Magic™ Human CaMK2γ(CAMK2G) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CAMK2G

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

cisR; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Intellectual Developmental Disorder, Autosomal Dominant 59 and Autism. Among its related pathways are Calmodulin induced events and Signaling downstream of RAS mutants

Gene ID

818

UniProt ID

Q13555

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The CAMK2G gene in humans encodes the calcium/calmodulin-dependent protein kinase type II gamma chain enzyme. This gene's product is a member of the Ca(2+)/calmodulin-dependent protein kinase subfamily as well as the Serine/Threonine protein kinase family. Multiple facets of glutamatergic synapses' plasticity depend on calcium signaling. The four chains that make up the enzyme in mammalian cells are delta, gamma, beta, and alpha. A gamma chain is the gene's byproduct. To date, six distinct isoforms have been identified from six alternatively spliced variants. Although other alternative splice variants encoding distinct isoforms have been reported, it is unknown what their full length composition is. The customized CAMK2G stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Ronald

The CAMK2G KD cell line has become a crucial tool in our lab, helping us gain deeper insights into angiogenesis and related processes. Nov 07 2020

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chat Thomas

As a researcher in vascular biology, I can confidently say that the CAMK2G KD cell line is a fantastic tool. It has significantly improved the accuracy and precision of our experiments. Jul 30 2021

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FAQ

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Published Data

Fig.1 CAMK2G bound and phosphorylated ITPKB at serine 174 to activate ITPKB in cisplatin-resistant ovarian cancer cells.

In cisR cells, CAMK2G interacts with ITPKB. cisR cell myc-pull-down samples were subjected to LC-MS/MS analysis. Verification of CAMK2G's interaction with ITPKB by reverse co-immunoprecipitation. There was overexpression of Myc-CAMK2G and/or Flag-ITPKB in the cisR cells.

Ref: Li, Jie, et al. "ROS-regulated phosphorylation of ITPKB by CAMK2G drives cisplatin resistance in ovarian cancer." Oncogene 41.8 (2022): 1114-1128.

Pubmed: 35039634

DOI: 10.1038/s41388-021-02149-x

Research Highlights

This study provides a potential treatment strategy for cisplatin resistance by revealing a critical kinase network comprising CAMK2G and ITPKB for ROS sensing and scavenging in ovarian cancer cells to maintain redox equilibrium.
Li, Jie, et al. "ROS-regulated phosphorylation of ITPKB by CAMK2G drives cisplatin resistance in ovarian cancer." Oncogene 41.8 (2022): 1114-1128.
Pubmed: 35039634 DOI: 10.1038/s41388-021-02149-x

These findings suggest that CAMK2G is involved in MF, and that blocking CAMK2G could be a novel way to treat the disease in cases where JAK1/2 inhibition is ineffective.
Miyauchi, Masashi, et al. "CAMK2G is identified as a novel therapeutic target for myelofibrosis." Blood Advances 6.5 (2022): 1585-1597.
Pubmed: 34521112 DOI: 10.1182/bloodadvances.2020003303