mProX™ Human CAMK2D Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1053	Magic™ Human CaMK2δ(CAMK2D) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CAMK2D

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MKN28; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research

Related Diseases

Dilated Cardiomyopathy and Cardiomyopathy, Dilated, 1Dd. Among its related pathways are Calmodulin induced events and Signaling downstream of RAS mutants

Gene ID

817

UniProt ID

Q13557

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human CAMK2D gene encodes the enzyme calcium/calmodulin-dependent protein kinase type II delta chain. This gene's product is a member of the Ca2+/calmodulin-dependent protein kinase subfamily as well as the serine/threonine protein kinase family. Multiple facets of glutamatergic synapses' plasticity depend on calcium signaling. The enzyme in mammalian cells is made up of four distinct chains: delta, gamma, beta, and alpha. A delta chain is the gene's product. To date, three distinct isoforms have been identified from four alternatively spliced transcript variants. This chain's distinct isoforms exhibit various expression patterns. The customized CAMK2D stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Joseph

This mouse CAMK2D cell line is the best choice for laboratories wishing to minimise troubleshooting time and maximise data and experimental volumes. Oct 26 2021

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chat Laura

The ideal CAMK2D overexpression cell line, often used in our laboratories. Mar 27 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Restoring the expression of CAMK2D interfered with miR-135b-related functions and remodeled EMT process.

MiR-135b mimics and a CAMK2D expression plasmid devoid of the miR-135b target area were co-transfected into GC cells. Western blot study of the expression of CAMK2D following co-transfection for 48 hours.

Ref: Huangfu, Longtao, et al. "MicroRNA-135b/CAMK2D Axis contribute to malignant progression of gastric cancer through EMT process remodeling." International journal of biological sciences 17.8 (2021): 1940.

Pubmed: 34131397

DOI: 10.7150/ijbs.58062

Research Highlights

These findings showed that miR-146a is essential for preserving cartilage homeostasis. Inhibiting MiR-146a in chondrocytes may be a useful treatment tactic to reduce OA.
Zhang, Xudong, et al. "miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2." Cell death & disease 8.4 (2017): e2734-e2734.
Pubmed: 28383548 DOI: 10.1038/cddis.2017.146

These findings show that the EMT process remodeling driven by the miR-135b/CAMK2D axis drives the progression of GC, implying that miR-135b may be used as a potential therapeutic target and prognostic marker for GC patients.
Huangfu, Longtao, et al. "MicroRNA-135b/CAMK2D Axis contribute to malignant progression of gastric cancer through EMT process remodeling." International journal of biological sciences 17.8 (2021): 1940.
Pubmed: 34131397 DOI: 10.7150/ijbs.58062