mProX™ Human BRSK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1044	Magic™ Human BRSK2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

BRSK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CCL13; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Infectious Research; Reproductive Research

Related Diseases

Autosomal Dominant Non-Syndromic Intellectual Disability and Carrion's Disease. Among its related pathways are Sweet Taste Signaling and Neuroscience

Gene ID

9024

UniProt ID

Q8IWQ3

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The gene BRSK2 (BR Serine/Threonine Kinase 2) codes for proteins. Carrion's disease and autosomal dominant non-syndromic intellectual disability are diseases linked to BRSK2. DNA damage and Sweet Taste Signaling are two of its associated mechanisms. Serine/threonine protein kinase that is essential for insulin production, cell cycle progression, and neuron polarization and axonogenesis. serves as a major regulator of cortical neuron polarization after being phosphorylated and activated by STK11/LKB1, most likely through mediating the phosphorylation of microtubule-associated proteins. The customized BRSK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Rebecca

The mouse BRSK2 KO cell line is easy to use, and the provided protocols are clear and straightforward. Its competitive pricing compared to other similar products on the market makes it an attractive option for researchers on a budget. Oct 16 2022

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chat Sarah

The BRSK2 KO cell line is approximately lower than other brands of reagents and I got similar results. Apr 12 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Activation of BRSK1 and BRSK2 mutants by LKB1.

Wild-type BRSK2 (WT) or BRSK2 carrying the T174A or T174E mutation, wild-type BRSK1 or BRSK1 carrying the G353A mutation, or wild-type BRSK2 or BRSK2 carrying the G310A mutation were transfected into CCL13 cells. The stated dose of adenovirus expressing LKB1 (10 pfu/cell in A) was used to infect cells, and the LNR peptide assay was used to quantify the activity of BRSK1 and BRSK2 in immune complexes.

Ref: Bright, Nicola J., David Carling, and Claire Thornton. "Investigating the regulation of brain-specific kinases 1 and 2 by phosphorylation." Journal of Biological Chemistry 283.22 (2008): 14946-14954.

Pubmed: 18339622

DOI: 10.1074/jbc.M710381200

Research Highlights

By functionally linking the BRSK2 and BRSK1 kinases to mTOR and NRF2 signaling, the data shed light on these kinases. NRF2 in human disease may be therapeutically targeted via this signaling axis.An accompanying primary Person interview with the paper's primary author is available here.
Tamir, Tigist Y., et al. "Gain-of-function genetic screen of the kinome reveals BRSK2 as an inhibitor of the NRF2 transcription factor." Journal of cell science 133.14 (2020): jcs241356.
Pubmed: 32546533 DOI: 10.1242/jcs.241356

These findings imply that population demographic and adaptive histories, as well as individual ancestry, genuinely affect DNA methylation profiles and that variation in DNA methylation diversity is a source of heterogeneity in human populations at macro and microgeographical scales.
Giuliani, Cristina, et al. "Epigenetic variability across human populations: a focus on DNA methylation profiles of the KRTCAP3, MAD1L1 and BRSK2 genes." Genome biology and evolution 8.9 (2016): 2760-2773.
Pubmed: 27503294 DOI: 10.1093/gbe/evw186