mProX™ Human BRSK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1043	Magic™ Human BRSK1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

BRSK1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HeLa; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Premature Menopause. Among its related pathways are Neuroscience and DNA Damage.

Gene ID

84446

UniProt ID

Q8TDC3

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The gene BRSK1 (BR Serine/Threonine Kinase 1) codes for proteins. Among the illnesses linked to BRSK1 is premature menopause. Neuroscience and DNA Damage are two connected mechanisms. Serine/threonine protein kinase that is essential for centrosome duplication and neuronal polarization. serves as a major regulator of cortical neuron polarization after being phosphorylated and activated by STK11/LKB1, most likely through mediating the phosphorylation of microtubule-associated proteins. The customized BRSK1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Deborah

The performance of the human BRSK1 cell line was consistent, and the company's support team was responsive and helpful throughout the process. Sep 15 2022

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chat Kimberly

I have tested the BRSK1 cell line from Creative Biolabs as one of the most reliable and versatile options available. Mar 09 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 BRSK1-mediated regulation of cisplatin sensitivity is dependent on its enzymatic activity.

Representative cisplatin dose-response curves in cisplatin-resistant and normal HeLa cells transduced with shCtrl or shBRSK1 (targeting 3' UTR), then infected with a plasmid expressing either the enzymatically inactive mutant BRSK1-K59A or wild-type BRSK1.

Ref: Liu, Guo, et al. "BRSK1 confers cisplatin resistance in cervical cancer cells via regulation of mitochondrial respiration." Journal of Cancer Research and Clinical Oncology (2023): 1-13.

Pubmed: 37140697

DOI: 10.1007/s00432-023-04821-z

Research Highlights

Because they participate in inflammation and apoptosis, urine and peripheral blood miR-195-5p, miR-192-3p, and miR-328-5p, together with their target genes PPM1A, RAB1A, and BRSK1, may be possible biomarkers for membranous nephropathy.
Zhou, Guangyu, et al. "Both peripheral blood and urinary miR-195-5p, miR-192-3p, miR-328-5p and their target genes PPM1A, RAB1A and BRSK1 may be potential biomarkers for membranous nephropathy." Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 25 (2019): 1903.
Pubmed: 30865617 DOI: 10.12659/MSM.913057

The results of this work show that the TSG genes BRSK1 and EGR1 carry frameshift mutations in GC and MSI-H CRC, together with mutational ITH, which may be characteristics of GC and CRC with MSI-H. These findings imply that TSG inactivation in CRC and GC may be a mechanism by which frameshift mutations in EGR1 and BRSK1 contribute to carcinogenesis.
Choi, Eun Ji, et al. "Putative tumor suppressor genes EGR1 and BRSK1 are mutated in gastric and colorectal cancers." Oncology 91.5 (2016): 289-294.
Pubmed: 27677186 DOI: 10.1159/000450616