mProX™ Human AURKB Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1031	Magic™ Human AurB(AURKB)/INCENP in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

AURKB

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Immunology Research; Digestive and Renal Research; Reproductive Research

Related Diseases

Nk-Cell Enteropathy and Polyploidy. Among its related pathways are Cell Cycle, Mitotic and EML4 and NUDC in mitotic spindle formation

Gene ID

9212

UniProt ID

Q96GD4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

One protein that aids in the mitotic spindle's adhesion to the centromere is called aurora kinase B. Kinases and phosphatases control chromosomal segregation during both mitosis and meiosis. Microtubules and the Aurora kinases form an association during chromosomal segregation and mobility. Whereas Aurora kinase A localizes to centrosomes, Aurora kinase B localizes to microtubules close to kinetochores, more especially, to the specialized microtubules known as K-fibers. Overexpression of these enzymes results in an uneven distribution of genetic information in malignant cells, a condition known as aneuploid cells. The customized AURKB stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Paddy

It was instrumental in our study, allowing us to specifically target AURKB proteins and investigate their role in disease. Jun 28 2023

chat Verified Customer

chat Sebastiane

This AURKB cell line is sensitive and specific. I definitely recommend this cell line. Sep 19 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 AURKB expression in cell line panel and drug response to barasertib.

Although at varied levels, overall expression of MYCN/MYC and AURKB was confirmed by western immunoblotting. All cell lines were found to contain TP53, with the exception of SK-N-AS and LAN-1, which have homozygous deletions of exons 10 and 11 and a harmful TP53 mutation (546C>A), respectively.

Ref: Bogen, Dominik, et al. "Aurora B kinase is a potent and selective target in MYCN-driven neuroblastoma." Oncotarget 6.34 (2015): 35247.

Pubmed: 26497213

DOI: 10.18632/oncotarget.6208

Research Highlights

This work suggests that AURKB is a good target for therapy in the treatment of gastric cancer since it shows that AURKB may enhance gastric carcinogenesis by epigenetically increasing CCND1 expression.
Nie, Min, et al. "AURKB promotes gastric cancer progression via activation of CCND1 expression." Aging (Albany NY) 12.2 (2020): 1304.
Pubmed: 31982864 DOI: 10.18632/aging.102684

AURKB levels are overexpressed in BRCA and are associated with a poor survival rate. Reversine modulates the aurora B, which in turn leads to the anti-growth action in BRCA cell lines, particularly for TNBC.
Huang, Di, et al. "Relation of AURKB over-expression to low survival rate in BCRA and reversine-modulated aurora B kinase in breast cancer cell lines." Cancer cell international 19.1 (2019): 1-13.
Pubmed: 31244554 DOI: 10.1186/s12935-019-0885-z