mProX™ Human ANO2 Stable Cell Line

Product Information

Target Family

Other Targets

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;NIH-3T3

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

Immunology Research

Related Diseases

Von Willebrand Disease, Type 3; Gnathodiaphyseal Dysplasia

Gene ID

Human:57101

UniProt ID

Human:Q9NQ90

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The applications of ANO2 include its influence on thermochemical and vibrational properties in coordination compounds, its role in the development of multiple sclerosis (MS) and preclinical neuroaxonal damage, its potential as a prognostic marker in cutaneous melanoma, and its involvement in the localization and survival of photoreceptor cells. ANO2 has been studied in the context of noncovalent interaction networks, seroreactivity against EBV antigens, pathomics signatures and genomics, and the bonding of [AnO2](2+) compounds. These studies have provided insights into the stability of actinyl compounds, the pathogenesis of MS, prognostic prediction in melanoma patients, and the biogenesis of membrane proteins in photoreceptor cells.

Protocols

Please visit our protocols page.

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FAQ

chat Cameron Davis (Verified Customer)

What is the function of ANO2 in olfactory sensory neurons? Jul 02 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

ANO2 acts as a calcium-activated chloride channel in olfactory sensory neurons, potentially mediating olfactory amplification. Jul 02 2023

chat Taylor Miller (Verified Customer)

How is ANO2 regulated and activated? Apr 05 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

ANO2 is regulated by cytoplasmic Ca2+ and post-translational modifications, and interacts with membrane lipids and protein partners for its activation. Apr 05 2020

Published Data

Fig.1 Overexpression of ANO2 in NIH-3T3 cells.

ANO2 expression was assessed through RT-PCR data, revealing transcription in the thalamus with β-actin utilized as a reference transcript. Subsequently, ANO2 protein expression in the thalamus was shown in the Western blotting data. A broader ANO2 band, indicative of different glycosylation levels, was observed in cells overexpressing ANO2.

Ref: Ha, Go Eun, et al. "The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons." Nature communications 7.1 (2016): 13791.

Pubmed: 27991499

DOI: 10.1038/ncomms13791

Research Highlights

Rajapaksha, Harindu. et al. "Three-Dimensional Noncovalent Interaction Network within [NpO2Cl4] 2-Coordination Compounds: Influence on Thermochemical and Vibrational Properties." Inorganic chemistry, 2023.
Noncovalent interactions (NCIs) have been shown to significantly impact the stability and chemical characteristics of pentavalent and hexavalent actinyl (AnO2/2+/0/,àí) complexes. These interactions play a crucial role in determining the reactivity, solubility, and potential toxicity of these complexes. NCIs can occur between the AnO2 moiety and various ligands, including anions, cations, and solvent molecules. The strength and orientation of these interactions can be influenced by factors such as steric hindrance and electrostatics. Understanding NCIs is vital for understanding the behavior and potential applications of actinyl compounds.
Rajapaksha, Harindu. et al. "Three-Dimensional Noncovalent Interaction Network within [NpO2Cl4] 2-Coordination Compounds: Influence on Thermochemical and Vibrational Properties." Inorganic chemistry, 2023.
Pubmed: 37816161 DOI: 10.1021/acs.inorgchem.3c02502

Jons, Daniel. et al. "Seroreactivity against lytic, latent and possible cross-reactive EBV antigens appears on average 10 years before MS induced preclinical neuroaxonal damage." Journal of neurology, neurosurgery, and psychiatry, 2023.
Previous research has shown a potential link between multiple sclerosis (MS) and presymptomatic axonal injury following an Epstein-Barr virus (EBV) infection. However, in order to accurately confirm this association, further studies need to be conducted that cover a wide range of time before clinical symptoms appear, while also considering both active and dormant forms of EBV and any potential cross-reacting antigens. These findings could provide a better understanding of the relationship between MS and EBV.
Jons, Daniel. et al. "Seroreactivity against lytic, latent and possible cross-reactive EBV antigens appears on average 10 years before MS induced preclinical neuroaxonal damage." Journal of neurology, neurosurgery, and psychiatry, 2023.
Pubmed: 37802637 DOI: 10.1136/jnnp-2023-331868