mProX™ Human AKT3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1025	Magic™ Human AKT3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

AKT3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCC; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Reproductive Research

Related Diseases

Megalencephaly-Polymicrogyria-Polydactyly-Hydrocephalus Syndrome 2 and Megalencephaly-Capillary Malformation-Polymicrogyria Syndrome

Gene ID

10000

UniProt ID

Q9Y243

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Instructions for producing a protein that is highly active in the nervous system are provided by the AKT3 gene. One of the main regulators of the PI3K-AKT-mTOR chemical signaling pathway is the AKT3 protein. Numerous essential cell processes, such as the synthesis of new proteins, cell division and development, and cell survival, are influenced by this signaling. The proper development of numerous bodily organs, including the brain, depends on the PI3K-AKT-mTOR pathway. Research indicates that brain size is mostly determined by the AKT3 protein. The customized AKT3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Eddy

Very good AKT3 cell line for drug discovery. Apr 26 2023

chat Verified Customer

chat Bill

Reliable and repeatable throughout its use. Mar 22 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 AKT3 expression is inversely correlated to miR-122 levels in HCC cell lines.

Several HCC cell lines were used to evaluate the AKT3 transcript level standardized to its expression in normal liver (right Y axis) and normalized miR-122 expression (left Y axis).

Ref: Nassirpour, Rounak, Pramod P. Mehta, and Min-Jean Yin. "miR-122 regulates tumorigenesis in hepatocellular carcinoma by targeting AKT3." PloS one 8.11 (2013): e79655.

Pubmed: 24244539

DOI: 10.1371/journal.pone.0079655

Research Highlights

AKT3, or v-akt murine thymoma viral oncogene homolog 3, is a member of the protein kinase B (PKB) family and encodes a 479 amino acid protein. It is found on chromosome 1q44. A common occurrence in 1q44 deletion syndrome patients with dysmorphic traits, intellectual impairment, and microcephaly is this gene.
Gai, Dayu, et al. "Phenotypes of AKT3 deletion: a case report and literature review." American Journal of Medical Genetics Part A 167.1 (2015): 174-179.
Pubmed: 25424989 DOI: 10.1002/ajmg.a.36710

An essential function of the PKB/Akt/phosphatidylinositol 3-kinase (PI3K) pathway is to control cell survival, proliferation, and metabolism.
Wang, Ling, et al. "Akt3 is responsible for the survival and proliferation of embryonic stem cells." Biology Open 6.6 (2017): 850-861.
Pubmed: 28483982 DOI: 10.1242/bio.024505