mProX™ Human AKT1 Stable Cell Line

Product Information

Target Protein

AKT1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

EBC1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Proteus Syndrome and Cowden Syndrome 6. Among its related pathways are Hepatocyte growth factor receptor signaling and PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Gene ID

207

UniProt ID

P31749

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The AKT1 gene codes for the production of the AKT1 kinase protein. This protein is present in many different types of cells throughout the body and is essential to numerous signaling pathways. AKT1 kinase signaling seems to be necessary for the correct growth and operation of the nervous system. Research has indicated that AKT1 kinase plays a part in memory formation, neuronal survival, and inter-neuronal communication. The AKT1 gene is a member of the oncogenes gene class. Oncogenes have the ability to alter and turn healthy cells malignant. The customized AKT1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Li Zhang

The AKT1 cell line is very good to use and is of high quality and safety. Aug 21 2023

chat Verified Customer

chat Richard

We obtained good data in downstream analysis using this AKT1 cell line. Mar 14 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Differential sensitivity of human cancer cells to different classes of AKT inhibitors.

AKT inhibitors' impact on AKT and AKT protein substrate phosphorylation. The stated doses of MK2206 and GSK690693 (top) or GDC0068 (bottom) were applied to EBC1 cells for a duration of 24 hours. Lysed treated cells were subjected to immunoblotting using the designated antibodies. Using image densitometry, the phosphorylation of GSK3β, BAD, and PRAS40 was measured (middle).

Ref: Vivanco, Igor, et al. "A kinase-independent function of AKT promotes cancer cell survival." Elife 3 (2014): e03751.

Pubmed: 25551293

DOI: 10.7554/eLife.03751

Research Highlights

It is widely accepted that Akt isoforms promote cancer by increasing cell proliferation and suppressing apoptosis in cancer cells and the tissues around tumors. Interestingly, research also shows that the Akt pathway is critical for tumor perfusion and metastasis, as well as for maintaining the integrity of the endothelium barrier and preventing aberrant vascular permeability.
Alwhaibi, Abdulrahman, et al. "The unconventional role of Akt1 in the advanced cancers and in diabetes-promoted carcinogenesis." Pharmacological research 145 (2019): 104270.
Pubmed: 31078742 DOI: 10.1016/j.phrs.2019.104270

The results show that resveratrol mitigates the unanticipated potential for metastasis brought on by anti-AKT therapy, indicating that resveratrol supplementation to an anti-AKT treatment regimen may offer additional assistance in preventing EMT.
Li, Chia-Wei, et al. "AKT1 inhibits epithelial-to-mesenchymal transition in breast cancer through phosphorylation-dependent Twist1 degradation." Cancer research 76.6 (2016): 1451-1462.
Pubmed: 26759241 DOI: 10.1158/0008-5472.CAN-15-1941