mProX™ Human ADGRG2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 Optimization of ADGRG2 Stachel peptide p15 for higher potency.
Dose-curves of p15, p15 mutants T1V, F3Tyr(Me), F3Phe(4-Me), F3(1-Nal), T1V/F3(1-Nal), and T1V/F3Phe(4-Me). HEK293 cells transfected with ADGRG2-ΔGPS-β were stimulated by increasing concentrations of p15 or p15 mutants.
Ref: Sun, Y., et al. Optimization of a peptide ligand for the adhesion GPCR ADGRG2 provides a potent tool to explore receptor biology. Journal of Biological Chemistry. 2021, 296.
Pubmed: 33303626
DOI: 10.1074/jbc.RA120.014726
Research Highlights
Obstructive azoospermia is primarily caused by congenital vas deferens absence (CAVD). CFTR and ADGRG2 mutations are to blame for this condition.
Yuan, P., et al. Expanding the phenotypic and genetic spectrum of Chinese patients with congenital absence of vas deferens bearing CFTR and ADGRG 2 alleles. Andrology. 2019, 7(3), 329-340.
Pubmed:
30811104
DOI:
10.1111/andr.12592
When combined with a Gs trimer, apo-ADGRG2 is a crucial membrane receptor for preserving male fertility. Dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate, and deoxycorticosterone were all identified as potential ligands of ADGRG2, while the formation of two kinks, which determined the active state, was determined by the two kinks.
Lin, H., et al. Structures of the ADGRG2-Gs complex in apo and ligand-bound forms. Nature Chemical Biology. 2022, 18(11), 1196-1203.
Pubmed:
35982227
DOI:
10.1038/s41589-022-01084-6
A crucial regulator of male fertility, the adhesion GPCR ADGRG2, sometimes referred to as GPR64, preserves ion/pH homeostasis and CFTR coupling. Due in part to the lack of identified endogenous ligands for ADGRG2 and the consequently constrained set of methods available to investigate ADGRG2 activity, the molecular basis of ADGRG2 function is little understood.
Sun, Y., et al. Optimization of a peptide ligand for the adhesion GPCR ADGRG2 provides a potent tool to explore receptor biology. Journal of Biological Chemistry. 2021, 296.
Pubmed:
33303626
DOI:
10.1074/jbc.RA120.014726
