Membrane Protein Overexpression Cell Line Development
Creative Biolabs offers membrane protein cell line development service to help you stably express your gene of interest constitutively or inducibly.
Stable transfection of cell lines is a time-consuming technique that usually requires the stable integration of recombinant DNA into the host cell genome. Antibiotic selection can identify stable integrant since the expression vector contains an antibiotic resistance gene. Overexpression a membrane protein may cause negative effects on cell growth, leading low expression level or even expression loss. Hence, it’s critical to make a decision whether to use a constitutive promoter or an inducible promoter to express the membrane protein of interest. To generate stable expression mammalian cell lines, lentiviruses are commonly utilized. They can be used to either down-regulate or up-regulate genes (through shRNA) (by using ORF of gene of interest).
Membrane Protein Constitutive Overexpression Cell Line Development
Creative Biolabs offers membrane protein constitutive overexpression cell line development service to help membrane protein drug discovery.
Learn More
Membrane Protein Inducible Overexpression Cell Line Development
Creative Biolabs has been a leader in the field of membrane protein and can provide you professional service for membrane protein inducible overexpression cell line development.
Learn MoreMembrane Protein Constitutive Overexpression Cell Line Development
Constitutive promoters are essential for membrane protein constitutive overexpression cell lines. They can maintain high gene expression for an extended period of time. Scientists have been identified numerous cellular/viral promoters that ensure sustained gene expression in various cell types. Creative Biolabs supplies weak and strong promoters for constitutive expression to suit your demands. Western blotting and radioligand binding assays can be used to determine the expression levels of each cell line. Another way to select for high-expressing cells is to employ fluorescence-activated cell sorting (FACS), which is commonly done with GFP. For example, by inserting an internal ribosome entry site (IRES) between the 5HT2c serotonin receptor and eGFP, scientists were able to link the two genes. As a result, high levels of fluorescence indicated significant amounts of 5HT2c serotonin receptor expression.
Fig.1. Strong and weak promoters for constitutive expression.
Membrane Protein Inducible Overexpression Cell Line Development
Inducible mammalian expression systems have been used to study the effects of specific gene products in living systems. It is preferable in these experiments to have no expressed protein in the uninduced state, as this allows physiological levels of the target protein to be synthesized after induction. As a result, expression systems are commonly characterized as having weak promoters that are tightly regulated. Creative Biolabs can provide tetracycline inducible system, cold inducible system, and DMSO inducible system for membrane protein inducible overexpression cell line development.
Fig.2. Inducible expression system.
Creative Biolabs offers stable cell line development service for membrane protein including GPCRs, Checkpoint Receptors, Ion Channels and many other targets for small molecule and biologics screening or profiling. With years of experience, we have gained remarkable success in generating stable expression cell lines. Please feel free to contact us to learn more information.
