HPV 74 L1+L2 Secreted Alkaline Phosphatase (SEAP) Pseudovirus
[CAT#: S01YF-0123-YF150]
- Product Category:
- Membrane Protein Tools
- Subcategory:
- Pseudovirus
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Information
Product Overview
HPV 74 pseudovirus (PsV) is made up of the major capsid protein L1 and the minor capsid protein L2. This PsV product is produced in 293FT cells transfected with plasmid expressing L1 and L2 protein and plasmid containing the reporter (Secreted Alkaline Phosphatase (SEAP)) gene. The product can be used in HPV 74 neutralization assay; HPV 74 antibody or vaccine discovery and development; mimic HPV 74 virus-like particles. The pseudovirus product is for research use only.
Organism
HPV 74
Virus Species
Human papillomavirus; HPV
Genotype
74
Packaging Proteins
L1; L2
Packaging System
293FT cells
Reporter
Secreted Alkaline Phosphatase (SEAP)
Size
1 mL; 2 mL; 5 mL; 10 mL
Formulation
DMEM complete medium
Research Area
Cancer Research; Infectious Research
Related Diseases
Cervical intraepithelial neoplasia (low risk)
Highlights
Validated
Quality Control
Titer Determination Method
Reed-Muench method
Titer Determination Detailed Protocol
1. Conduct 10-fold serial dilution of pseudovirus; add 100 uL diluted pseudovirus to each well of 96-well plate; and 6 repeats for each dilution;
2. Prepare 293FT suspension; add 3x10^4 cells to each well; and culture for 72 hours;
3. Analyze the results with SEAP kit.
2. Prepare 293FT suspension; add 3x10^4 cells to each well; and culture for 72 hours;
3. Analyze the results with SEAP kit.
Titer Unit
TCID50/mL
Sterility Testing
Negative
Mycoplasma Testing
Negative
Product Properties
Application
1. Pseudovirus Neutralization Assay
2. Antibody Discovery and Development
3. Vaccine Discovery and Development
4. Mimic Virus-Like Particles
2. Antibody Discovery and Development
3. Vaccine Discovery and Development
4. Mimic Virus-Like Particles
Handling Notes
1. Though pseudovirus particles has no pathogenicity and cannot replicate, the assays should be carried out carefully in a Biosafety Level 2 or higher-level laboratory with a biosafety cabinet.
2. Serum samples from animals or humans should be inactivated in a water bath at 56°C for 30 min before being tested.
3. Please avoid freez/thaw cycles, which would influence the titer of the pseudovirus product.
4. The product is for Research Use Only.
2. Serum samples from animals or humans should be inactivated in a water bath at 56°C for 30 min before being tested.
3. Please avoid freez/thaw cycles, which would influence the titer of the pseudovirus product.
4. The product is for Research Use Only.
Shipping
Dry ice
Storage
Store at -80°C. Avoid freeze/thaw cycles.
Protocols
Please visit our protocols page.
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