Total Internal Reflection Fluorescence Microscope (TIRF) Imaging Service

Introduction

Total Internal Reflection Fluorescence Microscopy (TIRF) is a cutting-edge imaging technique that offers unparalleled sensitivity and resolution for studying events close to the cell membrane. By utilizing an evanescent wave, TIRF selectively illuminates a thin section of the specimen adjacent to the glass-water interface, thereby reducing background fluorescence and enhancing the signal-to-noise ratio. This unique capability makes TIRF an invaluable tool for researchers aiming to delve deeper into cellular processes at the membrane level.

Protein analysis in single cells. (Yang, 2011) Fig.1 Schematic diagram of total internal reflection fluorescence microscopy imaging of a cell¹.

Applications

Insulin Granule Dynamics: TIRF has been employed to study insulin granule dynamics in human β-cells, providing insights into the behavior of these granules close to the cell membrane.
Cryogenic Cellular Imaging: A waveguide-based TIRF microscope has been developed to observe cells under cryogenic conditions, expanding the potential applications of TIRF in cellular biology.
Microtubule Dynamics: The technique has been adapted to simultaneously image microtubule-associated proteins and dynamic microtubules, offering a comprehensive view of cellular scaffolding.
Ion Channel Activity: TIRF microscopy has been used to correlate the lateral mobility and activity of individual ion channels in lipid membranes.

Service Process

1. Discuss the Project with the Customer and Sign the Project Contract: Before initiating the imaging process, it's essential to understand the client's requirements and set clear expectations.

2. Sample Preparation: The specimen is prepared on a glass coverslip, ensuring its proximity to the interface.

3. TIRF Imaging: Using specialized TIRF microscopes, the sample is illuminated, and high-resolution images are captured.

4. Data Analysis: The obtained images are analyzed using advanced software to extract meaningful insights.

5. Report Generation: A comprehensive report detailing the findings is prepared and shared with the client.

Creative Biolabs' Total Internal Reflection Fluorescence Microscope (TIRF) Imaging Service

At Creative Biolabs, we pride ourselves on offering state-of-the-art TIRF imaging services. Our team of experts is equipped with the latest TIRF microscopes and software to ensure that our clients receive the highest quality images. We understand the intricacies of TIRF imaging and are committed to delivering results that exceed expectations. Whether you're studying cellular dynamics or molecular interactions, Creative Biolabs is your trusted partner for all your TIRF imaging needs.

For more information, please contact us.

FAQs

Q1: What is the advantage of TIRF over traditional fluorescence microscopy?

A: TIRF offers superior z-axis resolution, allowing for selective illumination of specimens close to the cell membrane. This reduces background fluorescence and enhances image clarity.

Q2: Can TIRF be used for live-cell imaging?

A: Absolutely! TIRF is well-suited for live-cell imaging, especially for studying dynamic processes at the cell membrane.

Q3: How do I prepare my samples for TIRF imaging?

A: Samples should be prepared on glass coverslips to facilitate the generation of the evanescent wave. It's crucial to ensure that the specimen is in close proximity to the glass-water interface.

Reference

Yang, Yong, et al. "Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signal transduction." Chinese Science Bulleti 56 (2011): 1063-1067.

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