Stochastic Optical Reconstrunction Microscopy (STORM) Imaging Service

Introduction

Stochastic Optical Reconstruction Microscopy (STORM) represents a paradigm shift in the realm of high-resolution fluorescence microscopy. Unlike traditional microscopy methods, STORM harnesses the power of photoswitchable fluorophores, enabling researchers to achieve molecular-scale resolution, a feat previously thought unattainable. This revolutionary technique has opened new vistas in the microscopic world, allowing for unparalleled insights into cellular structures and processes.

Fig.1 Schematics of the working principle and the setup of the stochastic optical reconstruction microscopy (STORM)¹.

Applications

The applications of STORM are vast and varied. From mapping the location and identities of chemical synapses across whole cells in mouse retinae to investigating the intricate organization of pathological aggregates in the human brain, STORM has proven invaluable. Furthermore, its ability to resolve subcellular structures, such as axons, myelin sheaths, and astrocytic processes, has provided groundbreaking insights into the intricate world of cellular biology. The technique has also been employed to study the nanoscale organization of chromatin structures in pathological tissue samples, offering a deeper understanding of cellular anomalies.

Sample Requirements

For optimal STORM imaging, meticulous sample preparation is paramount. This includes considerations for the type of neurons, whether in vitro or in vivo, conjugation of dyes to antibodies, and immunofluorescence labeling6. Ensuring the sample's integrity and appropriate labeling is crucial for the acquisition and processing of high-quality STORM data.

Service Process

1. Discuss the project with the customer and sign the project contract.

2. Sample collection and verification.

3. Sample preparation, including dye conjugation and immunofluorescence labeling.

4. STORM imaging acquisition.

5. Data processing and analysis.

6. Report generation and delivery to the customer.

7. Post-project consultation and feedback.

Creative Biolabs' Stochastic Optical Reconstruction Microscopy (STORM) Imaging Service

At Creative Biolabs, we pride ourselves on offering state-of-the-art STORM imaging services. Our team of dedicated professionals, equipped with cutting-edge technology, ensures that our clients receive the most accurate and detailed imaging results. We understand the intricacies of STORM and are committed to providing unparalleled service quality. From the initial consultation to the final report delivery, our focus remains on achieving excellence and ensuring customer satisfaction.

For more information, please contact us.

FAQs

Q1: What are the advantages of STORM over other microscopy methods?

A: STORM offers molecular-scale resolution, allowing for detailed insights into cellular structures that were previously unobservable with traditional microscopy methods.

Q2: How long does the STORM imaging process take?

A: The duration varies depending on the sample's complexity and the specific requirements of the project. However, our team at Creative Biolabs ensures timely delivery of results without compromising on quality.

Q3: Are there any potential challenges or limitations associated with STORM imaging?

A: While STORM offers unparalleled resolution, it requires meticulous sample preparation and the right conditions for optimal results. Additionally, certain dyes and antibodies are more conducive to STORM imaging than others.

Reference

1. Xia, Yifan, and Bingmei M. Fu. "Investigation of endothelial surface glycocalyx components and ultrastructure by single molecule localization microscopy: stochastic optical reconstruction microscopy (STORM)." The Yale Journal of Biology and Medicine 91.3 (2018): 257-266.