Stimulated Emmission Depletion (STED) Imaging Service

Introduction

Stimulated Emission Depletion (STED) Imaging is a groundbreaking super-resolution microscopy technique that has revolutionized the realm of cellular and molecular imaging. By surpassing the diffraction limit of conventional optical microscopy, STED offers unparalleled insights into the nanoscale intricacies of biological systems.

Fig.1 Stimulated emission depletion (STED) mechanism demonstrated with Alexa 647 fluorescence¹.

Applications

STED imaging has found a plethora of applications in the scientific community:

Neuroscience: STED has been instrumental in visualizing the dynamic morphology of dendritic spines of pyramidal neurons in living hippocampal slices.
Cell Biology: It has enabled the imaging of endocytic events at the plasma membrane of living cells.
Structural Biology: STED has been used to visualize cell division proteins in bacterial cells, specifically E. coli and B. subtilis.

Service Process

1. Discuss the project with the customer and sign the project contract.

2. Sample collection and preparation, ensuring the integrity and specificity of the target molecules.

3. Selection of appropriate fluorophores and labeling techniques.

4. Imaging using state-of-the-art STED microscopes, ensuring optimal resolution and minimal photobleaching.

5. Data analysis and interpretation, leveraging advanced computational tools.

6. Report generation and consultation with the customer, discussing the findings and their implications.

Creative Biolabs' Stimulated Emission Depletion (STED) Imaging Service

At Creative Biolabs, we pride ourselves on offering top-tier STED imaging services. Our team of experts, equipped with cutting-edge STED microscopes, ensures that our clients receive the most detailed and accurate imaging results. We understand the nuances of STED imaging and offer tailored solutions, ensuring that the unique requirements of each project are met. Our commitment to excellence and our emphasis on customer satisfaction make us a preferred choice for researchers and institutions worldwide.

For more information, please contact us.

FAQs

Q1: What is the resolution advantage of STED over conventional microscopy?

A: STED microscopy can achieve resolutions below 50 nm, a significant improvement over the ~200 nm limit of conventional optical microscopy.

Q2: Is STED suitable for live-cell imaging?

A: Yes, with careful sample preparation and optimized imaging conditions, STED can be used for live-cell imaging.

Q3: How does STED compare with other super-resolution techniques like STORM?

A: Both STED and STORM offer super-resolution capabilities, but the choice between them depends on the specific application and the user's expertise.

Q4: Are there any limitations to STED imaging?

A: While STED offers superior resolution, it requires specialized equipment and expertise. Additionally, the choice of fluorophores and potential photobleaching are considerations to keep in mind.

Reference

Valli, Jessica, and Jeremy Sanderson. "Super‐Resolution Fluorescence Microscopy Methods for Assessing Mouse Biology." Current Protocols 1.8 (2021): e224.

Note: All of our products are for Research Use Only (RUO). NOT intended for diagnostic, therapeutic or clinical use. We DO NOT offer patients any direct products or services. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.