Small Animal Live Imaging Service

Introduction

In the ever-evolving domain of scientific research, in vivo imaging of small animals has emerged as a transformative tool. This technique, which encompasses both bioluminescence and fluorescence modalities, allows researchers to visualize and track biological processes within living organisms, offering real-time insights into disease progression, drug response, and more.

Fig.1 Bioluminescence image of nude mice inoculated with HCT116 cells¹.

Applications of Small Animal Live Imaging in Scientific Research

The in vivo imaging of small animals, particularly using bioluminescence and fluorescence, has a myriad of applications:

• Disease Progression: Track the onset and progression of diseases in real-time within a living organism.
• Drug Development: Monitor the efficacy and distribution of novel therapeutic agents.
• Gene Expression: Observe the expression of specific genes by tagging them with luminescent or fluorescent markers.
• Cell Tracking: Monitor the migration and proliferation of cells, especially useful in stem cell research and oncology.

Pros and Cons of Small Animal Live Imaging in Scientific Research

Pros:

• Non-invasive technique allowing longitudinal studies without harming the animal.
• Real-time visualization of biological processes.
• Ability to multiplex by using different wavelengths for simultaneous tracking of multiple biological events.

Cons:

• Limited depth penetration, especially in fluorescence imaging.
• The need for external light sources in fluorescence can sometimes cause tissue auto-fluorescence.
• Bioluminescence requires the presence of a substrate, which might affect certain physiological processes.

Sample Requirements

For optimal results:

• Animals should be genetically modified to express luminescent or fluorescent markers, or these markers should be introduced externally.
• Ensure the animal's fur, if present, is cleared to reduce light scattering.
• Use appropriate anesthetics to reduce animal movement during imaging.

Service Process

1. Discuss the project with the customer and sign the project contract.

2. Understand the specific requirements and objectives of the imaging study.

3. Prepare the animal model, ensuring the presence of luminescent or fluorescent markers.

4. Conduct the imaging session, ensuring minimal external light interference.

5. Process and analyze the acquired images.

6. Provide the customer with comprehensive reports and insights derived from the imaging study.

Creative Biolabs' Small Animal Live Imaging Service

Creative Biolabs, a pioneer in the realm of scientific research, offers a cutting-edge Small Animal Live Imaging Service. Specializing in both bioluminescence and fluorescence techniques, our team ensures precise, high-resolution imaging tailored to your research needs. With a commitment to innovation, Creative Biolabs is your trusted partner in advancing the boundaries of in vivo imaging.

For more information, please contact us.

FAQs

Q1: What's the difference between bioluminescence and fluorescence imaging?

A: Bioluminescence is a naturally occurring phenomenon where light is produced by a chemical reaction within an organism, while fluorescence involves the absorption of light at one wavelength and its re-emission at another.

Q2: How deep can these imaging techniques penetrate?

A: While bioluminescence has a relatively deeper tissue penetration, fluorescence is limited by the need for external excitation light, which can be scattered or absorbed by tissues.

Q3: Are there any side effects on animals?

A: Both techniques are considered non-invasive. However, care is taken to ensure the well-being of the animals, especially when introducing external markers or substrates.

Reference

1. Pan, Yang, et al. "Nuclear Beclin 1 Destabilizes Retinoblastoma Protein to Promote Cell Cycle Progression and Colorectal Cancer Growth." Cancers 14.19 (2022): 4735.