jGCaMP8 Sensor Tools

Tracking brain activity in specific neurons and neural networks is typically done with genetically encoded calcium indicators (GECIs), which measure Ca²⁺-dependent fluorescence. In particular, the signal-to-noise ratio (SNR) for detecting Ca²⁺ entering neurons during neural activity has been improved for the green fluorescent protein (GFP)-based GCaMP sensors through iterative engineering. There are three types of jGCaMP8 sensors: jGCaMP8f (fast rise and fast decay), jGCaMP8m (fast rise and medium decay), and jGCaMP8s (fast rise, slow decay, and sensitive). All jGCaMP8 sensors can track individual spikes in neurons with spike frequencies up to 50 Hz and have fluorescence rise times that are nearly ten times faster than those of prior GCaMPs. Because jGCaMP8 sensors are more linear than earlier GCaMPs, strong deconvolution for spike extraction is possible. The jGCaMP8 sensors performed better across a variety of parameters important for imaging neuronal populations in vivo when tested in vivo on mice and flies. Creative Biolabs organized a professional team to provide our customers with jGCaMP8-related tools.