GRAB_eCB Sensor Tools

Retrograde lipid neuromodulators known as endocannabinoids (eCBs) are engaged in numerous physiologically significant processes. However, due in large part to a paucity of probes able to report real-time eCBs with enough spatiotemporal resolution, their release and dynamics in the brain are still completely unknown. Researchers used the human CB1 cannabinoid receptor and a circularly-permutated EGFP to create the GRAB_eCB2.0, a novel G protein-coupled receptor activation-based eCB sensor. When exposed to eCBs at physiological doses, GRAB_eCB2.0 demonstrated correct cell membrane trafficking, seconds kinetics, excellent specificity, and a robust fluorescence response. They easily noticed running-triggered and foot-shock-elicited eCB transients in the mouse hippocampus and amygdala by expressing GRAB_eCB2.0 in vivo. They noticed a spreading eCB wave following a calcium wave in the rat hippocampus utilizing GRAB_eCB2.0 in an epileptic model. In conclusion, GRAB_eCB2.0 is a potent novel probe to unravel eCB dynamics and release in both healthy and pathological settings. With years of experience, Creative Biolabs can provide top-quality GRAB_eCB-related tools. We are also dedicated to providing customized services to contribute to the success of your project.