GCaMP6m Sensor Tools
- Product Category:
- Membrane Staining and Imaging Reagents
- Subcategory:
- Genetically Encoded Sensors
A genetically encoded fluorescent Ca2+ indicator with high sensitivity and moderate degradation kinetics is called GCaMP6m. The following mutations exist in GCaMP6m in comparison to GCaMP5G: M378G, K379S, T381R, S383T, and R392G. A four-state model that describes how GCaMP6m modifies fluorescence in response to Ca2+ reveals that the main contributing mechanism is a Ca2+-dependent alteration in the chromophore protonation state brought on by a change in pKa. The protein experiences conformational changes that quantitatively account for the pKa shift by changing the electrostatic potential surrounding the chromophore when calmodulin binds to Ca2+ and interacts with the M13 peptide. The absolute pKa values for the Ca2+-free and Ca2+-saturated states of GCaMP6m determine its high signal-to-noise ratio. This process has significant ramifications for future enhancements to GCaMP6m and perhaps other biosensors with comparable designs. Creative Biolabs has been working on developing and optimizing GCaMP6m plasmids, viruses, and cell lines for our clients.
- Plasmid
- Cell line
- Virus
