GCaMP6f Sensor Tools

Product Category:: Membrane Staining and Imaging Reagents

Subcategory:: Genetically Encoded Sensors

The GCaMP6 sensors offer a similar baseline brightness compared to GCaMP5G and a 1.1-1.6-fold increase in dynamic range. The most sensitive sensor gave signals that were seven times greater for tiny quantities of action potentials. With sensitivity comparable to OGB1-AM, GCaMP6f is the quickest genetically encoded calcium indicator for cytoplasmic free calcium in neurons. It is made up of calmodulin, a circularly permuted green fluorescent protein, and the calmodulin-binding peptide M13. The following mutations exist in GCaMP6f in comparison to GCaMP5G: A317E, T381R, S383T, and R392G. Creative Biolabs can offer GCaMP6f-related products. The technicians at Creative Biolabs are available at all times to help clients with their projects and look for the best solution for each one.

Plasmid
Cell line
Virus

Creative Biolabs can offer plasmids expressed genetically encoded sensors.

Product Name	Cat#	Vector	Vector Type
GCaMP6f AVV Plasmid	S01YF-0623-KX122	pAAV
GCaMP6f Lentiviral Plasmid	S01YF-0623-KX123	pCDH	Mammalian Expression
Custom GCaMP6f Plasmid	S01YF-0623-KX124	Customized	Mammalian Expression

Creative Biolabs can provide customized genetically encoded sensor cell lines to support membrane protein drug screening and development.

Product Name	Cat#	Host Cell Type	Quantity (Cells)
GCaMP6f Stable CHO Cell Line	S01YF-0623-KX125	CHO	1×10⁶
GCaMP6f Stable HEK293 Cell Line	S01YF-0623-KX126	HEK293	1×10⁶
Custom GCaMP6f Stable Cell Line	S01YF-0623-KX127	Customized	1×10⁶

Creative Biolabs offers genetically encoded sensor lentiviral virus and AAV virus to meet special requirements.

Product Name	Cat#	Serotype	Reporter Gene	Titer Unit	Size
GCaMP6f-CaMKIIa AAV Virus	S01YF-0623-KX128		None	10¹² v.g./mL	50 μL
GCaMP6f-EF1a AAV Virus (DIO-GCaMP6f-P2A-nls)	S01YF-0623-KX129		tdTomato	10¹³ v.g./mL	50 μL
GCaMP6f-Syn AAV Virus (Flex-GCaMP6f)	S01YF-0623-KX130		None		50 μL
GCaMP6f-Syn AAV Virus	S01YF-0623-KX131		None		50 μL
GCaMP6f-CMV bGlobin AAV Virus	S01YF-0623-KX132		None	10¹³ v.g./mL	50 μL
GCaMP6f-Syn AAV Virus (mRuby2-GSG-P2A-GCaMP6f-WPRE-pA)	S01YF-0623-KX133		mRuby2	10¹³ v.g./mL	50 μL
GCaMP6f-CaMKIIa AAV Virus (GCaMP6f-P2A-nls)	S01YF-0623-KX134		tdTomato		50 μL
GCaMP6f-Syn AAV Virus (GCaMP6f-P2A-nls)	S01YF-0623-KX135		tdTomato		50 μL
GCaMP6f -Syn AAV Virus (GCaMP6f-WPRE)	S01YF-0623-KX136		None	10¹³ v.g./mL	100 μL
GCaMP6f -Syn AAV Virus (FLEX-GCaMP6f-WPRE)	S01YF-0623-KX137		None	10¹³ v.g./mL	100 μL
GCaMP6f -CAG AAV Virus (FLEX-GCaMP6f-WPRE)	S01YF-0623-KX138		None	10¹³ v.g./mL	100 μL
GCaMP6f -CAG Lentiviral Virus	S01YF-0623-KX139	None	None	10⁸ CFU/mL
GCaMP6f -CMV Lentiviral Virus	S01YF-0623-KX140	None	None	10⁸ CFU/mL
somaGCaMP6f2-hSyn AAV Virus (DIO-somaGCaMP6f2)	S01YF-0623-KX141	AAV9	None		100 μL
somaGCaMP6f1-hSyn AAV Virus (DIO-somaGCaMP6f1)	S01YF-0623-KX142	AAV9	None		100 μL
somaGCaMP6f2-hSyn AAV Virus	S01YF-0623-KX143	AAV9	None		100 μL

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact