GCaMP2 Sensor Tools
- Product Category:
- Membrane Staining and Imaging Reagents
- Subcategory:
- Genetically Encoded Sensors
The ability to measure Ca2+ dynamics in a physiologically realistic setting is made possible by the use of genetically encoded Ca2+ markers. A circularly permutated EGFP (cpEGFP)/M13/Calmodulin (CaM) fusion protein called GCaMP2, one of the most reliable indicators, has been utilized to successfully examine Ca2+ fluxes in vivo in the heart and vasculature of transgenic mice. CaM maintains the fluorophore in an ionized state comparable to that of EGFP in the brilliant state. Critical connections between the fluorophore and components of the fused peptides were validated by mutational analysis. The compact, pre-docked state of GCaMP2 in its Ca2+-free form, as revealed by solution scattering experiments, may provide a mechanistic explanation for the indicator's comparatively quick signaling kinetics. With extensive experience in the field of genetically encoded sensors, Creative Biolabs offers GCaMP2 plasmids, viruses, and cell lines for our clients.
- Plasmid
- Cell line
- Virus
| Product Name | Cat# | Serotype | Reporter Gene | Titer Unit | Size | Datasheet | Inquiry |
|---|---|---|---|---|---|---|---|
| Custom GCaMP2 Lentiviral Virus | S01YF-0623-KX102 | N/A | >10⁸ TU/mL | ||||
| Custom GCaMP2 AAV Virus | S01YF-0623-KX103 | >10¹² v.g./mL |
