Photoactivated Localization Microscopy (PALM) Imaging Service

Introduction

Photoactivation Localization Microscopy (PALM) is an advanced imaging technique that has revolutionized the realm of microscopy by enabling near-molecular resolution. By leveraging the unique properties of photoactivatable fluorescent proteins, PALM provides insights into cellular structures and processes that were previously obscured by the limitations of traditional microscopy.

Fig.1 PALM imaging of the actin cytoskeleton in HeLa cells¹.

Applications of PALM Imaging in Scientific Research

PALM imaging has found extensive applications in various scientific research areas. For instance, it has been instrumental in the quantitative analysis of sub-diffraction PALM images, providing insights into molecular interactions and the regulation of cellular signaling mechanisms. Additionally, PALM has been employed in the study of cellular substructures in live cells, offering unparalleled clarity and detail. The technology has also been pivotal in the exploration of super-resolution in single-cell imaging, enhancing the precision and understanding of cellular processes.

Pros and Cons of PALM Imaging Service in Scientific Research

Pros:

Achieves near-molecular resolution, surpassing traditional microscopy techniques.
Enables long-term live-cell imaging with reduced phototoxicity.
Provides a platform for high-speed imaging, capturing rapid cellular events.

Cons:

Requires specialized equipment and expertise.
Sample preparation can be intricate, especially for live-cell imaging.
Potential for photo-bleaching and other artifacts if not handled correctly.

Service Process

1. Discuss the project with the customer and sign the project contract.

2. Sample collection and preparation, ensuring the use of appropriate fluorescent proteins.

3. Calibration and setup of the PALM imaging system.

4. Image acquisition, leveraging advanced algorithms for optimal resolution.

5. Data analysis and interpretation, providing comprehensive insights into the sample.

6. Report generation and delivery to the customer, detailing the findings.

Creative Biolabs' PALM Imaging Service

At Creative Biolabs, we pride ourselves on offering state-of-the-art PALM imaging services. Our team of experts ensures that every step, from sample preparation to data analysis, is executed with precision and dedication. Leveraging the latest advancements in PALM technology, we strive to provide our clients with insights that drive scientific discovery forward.

For more information, please contact us.

FAQs

Q1: How does PALM achieve super-resolution?

A: PALM utilizes photoactivatable fluorescent proteins that can be switched on and off. By localizing individual molecules in successive frames, a high-resolution image is constructed.

Q2: Can PALM be used for live-cell imaging?

A: Yes, PALM is suitable for live-cell imaging, especially when using fluorophores that reduce phototoxicity.

Q3: What are the main challenges in PALM imaging?

A: Some challenges include sample drift, potential photo-bleaching, and the need for a specific chemical environment for optimal fluorophore blinking.

Reference

Endesfelder, Ulrike, et al. "Chemically induced photoswitching of fluorescent probes—a general concept for super-resolution microscopy." Molecules 16.4 (2011): 3106-3118.

Note: All of our products are for Research Use Only (RUO). NOT intended for diagnostic, therapeutic or clinical use. We DO NOT offer patients any direct products or services. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.