DiscoverMP™ Membrane Vesicles (Extracellular Vesicles; EVs) From Bone Marrow-derived Mesenchymal Stromal Cells (MSCs)
- Product Category:
- DiscoverMP™ Membrane-based Drug Delivery System
- Subcategory:
- Membrane Vesicles (Extracellular Vesicles)
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Lot Number
Product Information
Product Quality Control
The EVs are bacteria and fungi free, which can be used for sensitive cell cultures.
The EVs are mycoplasma free, which can be used for sensitive cell cultures.
Treatment of mouse macrophage cells (RAW264.7) with lipopolysaccharide (LPS) induces the formation of nitric oxide (NO) formation indicating an inflammatory reaction. Addition of EVs derived from Bone Marrow-derived Mesenchymal Stromal Cells (MSCs) significantly reduces NO secretion, indicating positive anti-inflammatory activity of the EVs.
Treatment of human fibroblasts (fHDF/TERT166) with Transforming Growth Factor beta (TGF-β1) induces the expression of alpha smooth muscle actin (α-SMA) indicating myofibroblast differentiation/activation, which is a key event in physiological and pathological tissue repair. Addition of EVs derived from Bone Marrow-derived Mesenchymal Stromal Cells (MSCs) significantly reduces α-SMA induction, indicating positive anti-fibrotic activity of the EVs.
Product Handling and Storage
Note: Thaw the EVs on ice, centrifuge before opening the tube to ensure that the solution is collected at the bottom of the tube. Then, mix carefully by pipetting up and down and aliquot for further use. Avoid freeze/thaw cycles as it may result in irreversible damage to the EVs.
Disclaimer: We cannot guarantee the activity if the EVs are not handled according to procedure.
After thawing, store the EVs at 4°C for a maximum of 1 day.
Protocols
Please visit our protocols page.
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Research Highlights
Börger, Verena, et al. "International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells: considerations for potential therapeutic agents to suppress coronavirus disease-19." Cytotherapy 22.9 (2020): 482-485.
Sengupta, Vikram, et al. "Exosomes derived from bone marrow mesenchymal stem cells as treatment for severe COVID-19." Stem cells and development 29.12 (2020): 747-754.
Casado-Díaz, Antonio, José Manuel Quesada-Gómez, and Gabriel Dorado. "Extracellular vesicles derived from mesenchymal stem cells (MSC) in regenerative medicine: applications in skin wound healing." Frontiers in Bioengineering and Biotechnology 8 (2020): 146.
