Humanized Murine Cancer Cell Screening Service

Checkpoint inhibitors combined with immunotherapy opened up new avenues for the treatment of cancer. Preclinical immunotherapy studies are increasingly using humanized mice, which are capable of expressing both human immune system and human malignancies. Creative Biolabs offers humanized murine cancer cells screening service to provide a platform for evaluating human specific agents.

There has been an increased interest in immune checkpoint inhibitors. Research on cancer has made use of genetically modified mice models. They are genetically modified to either express an oncogene or remove a tumor suppressor gene, which promotes the growth of tumors, potentially in a tissue-specific manner. We have created human target-expressing mice cancer cells. The models can be used to assess how well human-specific medicines that human target proteins produced on tumor cells work.

Mouse tumor cells model. (Joalland, et al., 2020) Fig.1. Mouse tumor cells model. ¹

Features of Humanized Murine Cancer Cells Screening Service

As a more affordable option to more intricate completely humanized models, consider utilizing engineered murine cells and engineered murine model combination models to save research resources.
Assess human-specific biological treatments in vivo in cases when a mouse ortholog is not accessible.
Examine how human ligands interact with receptors.

Applications of Humanized Murine Cancer Cells Screening Service

Efficacy of Anti-Immune Checkpoint Treatment

While PBMC-humanized mice engrafted with osteosarcoma tumors showed no effect from the antibody on tumor growth, it did partially limit the production of lung metastases by increasing the number of CD4⁺ and CD8⁺ T lymphocytes as well as the cytolytic activity of CD8⁺ T cells in the lungs.

Efficacy of Bispecific Antibodies

The T cell bispecific antibody CD20-TCB was found to have an anti-tumoral effect in HSC-humanized NSG mice engrafted with human B cell lymphoma. This effect was mediated by T cell recruitment to the tumor as well as resident T cell proliferation, as well as by the quick formation of stable T cell-tumor cell synapses inducing tumor cytotoxicity and cytokine synthesis.

Efficacy of Therapeutic Cancer Vaccines

Since the constitutive expression of HPV proteins E6 and E7 in tumors is linked to human papillomavirus (HPV)-oncogenesis, numerous clinical trials have examined the effectiveness of therapeutic vaccinations targeting E6 and E7.

Efficacy of Combination Treatments

Although there are insufficient representative preclinical models, Epstein-Barr virus (EBV) linked nasopharyngeal cancer is a highly inflammatory tumor type with lymphocyte infiltration and PD-L1 expression, making it an excellent candidate for immunotherapy.

To assist you in advancing the evaluation of your compound in immuno-oncology, Creative Biolabs offers humanized murine cancer cells screening service that is available for use in tumor investigations. These cells have been profiled with recognized immune checkpoint inhibitors of clinical significance. To assist you in achieving your objectives, our team can help choose the ideal model platform and provide relevant studies. Please contact us for more information.

Reference

Joalland, Noémie, and Emmanuel Scotet. "Emerging challenges of preclinical models of anti-tumor immunotherapeutic strategies utilizing Vγ9Vδ2 T cells." Frontiers in immunology 11 (2020): 992.

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