Structured Illumination Microscopy (SIM) Imaging Service

Introduction

Structured Illumination Microscopy (SIM) is a cutting-edge imaging technique that surpasses the traditional diffraction limit of optical microscopy. By employing sinusoidal-patterned illumination and subsequent post-processing, SIM extracts high-frequency components, enabling the acquisition of super-resolution images. This method offers a twofold improvement in resolution, reaching approximately 120 nm, making it a powerful tool for visualizing intricate cellular structures and interactions.

Fig.1 Structured illumination microscopy (SIM). The sample is excited by a grid-like patterned light with alternating bands of high and low intensity¹.

Applications

SIM has been instrumental in various research areas, particularly in the realm of cellular biology. It has facilitated the precise intracellular localization of nanomaterials, allowing scientists to not only pinpoint the exact positioning of these materials but also monitor their degradation over time. The enhanced resolution of SIM has been pivotal in observing changes in the size or shape of nanomaterials in real-time. Furthermore, SIM's capability for live-cell imaging makes it a preferred choice over other super-resolution techniques, which may pose challenges in this domain.

Pros and Cons

Pros:

Enhanced resolution: SIM offers a twofold improvement in resolution, allowing for detailed visualization of cellular structures.
Live-cell imaging: The technique's low illumination power is conducive to live-cell imaging without causing damage.
Versatility: SIM can be combined with other techniques, such as nanoscale secondary ion mass spectrometry and X-ray microscopy, for comprehensive analyses.

Cons:

Complexity: The method requires intricate setups and post-processing steps.
Limitations in thick samples: In samples where in-focus signals are overshadowed by out-of-focus signals, the advantages of SIM may be restricted.

Service Process

1. Discuss the project with the customer and sign the project contract.

2. Sample submission and verification.

3. Sample preparation and labeling.

4. SIM imaging acquisition.

5. Post-processing and data analysis.

6. Report generation and delivery to the customer.

7. Post-project consultation and feedback.

Creative Biolabs' Structured Illumination Microscopy (SIM) Imaging Service

At Creative Biolabs, we pride ourselves on offering state-of-the-art SIM imaging services. Our team of experts is equipped with the latest technology and is dedicated to providing high-quality, detailed images to advance your research endeavors. We understand the intricacies of SIM and ensure that each project is handled with utmost precision and care. With our services, you can be confident in obtaining reliable and insightful data for your research needs.

For more information, please contact us.

FAQs

Q1: How does SIM differ from traditional microscopy?

A: SIM employs sinusoidal-patterned illumination and post-processing to achieve super-resolution, surpassing the diffraction limit of traditional microscopy.

Q2: Is SIM suitable for live-cell imaging?

A: Yes, the high temporal resolution and low illumination power of SIM make it ideal for live-cell imaging.

Q3: What are the sample requirements for SIM?

A: Samples should be prepared to minimize scattering, and the fluorescent probes used should be compatible with the SIM technique. Proper mounting in suitable media is also essential.

Reference

Nieto-Garai, Jon Ander, et al. "Super-Resolution Microscopy to Study Interorganelle Contact Sites."International Journal of Molecular Sciences 23.23 (2022): 15354.

Note: All of our products are for Research Use Only (RUO). NOT intended for diagnostic, therapeutic or clinical use. We DO NOT offer patients any direct products or services. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.