mProX™ Human TSSK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX516	Magic™ Human TSSK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Kinase Cell Lines

Gene ID

Human:81629

UniProt ID

Human:Q96PN8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TSSK3, a testis-specific serine/threonine kinase, has been studied in various contexts. In tree shrews, TSSK3 is expressed during the reproductive season and is involved in spermatogenesis and sperm function regulation. It is specifically localized in the postacrosomal region and flagellum of tree shrew sperm. In mice, TSSK3 is essential for spermiogenesis and male fertility, as its absence leads to disorganization of testicular seminiferous epithelium, reduced sperm counts, and abnormal sperm morphology. TSSK3 is also implicated in male infertility and nonhormonal contraception. Additionally, a method called KinOrtho has been developed to map TSSK3 orthologs across different species, enabling the study of understudied kinases and their functions. Overall, TSSK3 plays important roles in reproductive function, sperm development, and male fertility.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Morgan Smith (Verified Customer)

How does FSH treatment affect TSSK3 expression in Sertoli cells? Aug 01 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

FSH treatment significantly improves the expression of TSSK3 in Sertoli cells, which may play a crucial role in the meiosis and mitosis of spermatogenic cells. Aug 01 2021

chat Taylor Garcia (Verified Customer)

Is TSSK3 involved in the process of spermiogenesis in other species? Jul 28 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Yes, TSSK3 and other Tssk family members are primarily expressed in the mature testis of species like the bay scallop Argopecten irradians, suggesting their role in spermiogenesis. Jul 28 2021

Published Data

Fig.1 An elevation in the expression of TSSK3 was observed, with its levels being increased in a dose-dependent fashion following FA supplementation.

In a dose-dependent manner, the expression of TSSK3 was increased by FA supplementation. Antibodies for TSSK3, GAPDH, or caspase three were probed on the blot for 24 hours at 4 °C. The bands were developed with HRP-conjugated secondary antibodies using ECL reagent, and the densities of the bands were quantified using ImageJ software. A representative Western blot is shown in the upper panel, while the average ratio of band intensities from three separate blots is displayed in the lower panel.

Ref: Junaid, Mohammed A., et al. "Folic acid supplementation dysregulates gene expression in lymphoblastoid cells-implications in nutrition." Biochemical and Biophysical Research Communications 412.4 (2011): 688-692.

Pubmed: 21867686

DOI: 10.1016/j.bbrc.2011.08.027

Research Highlights

Tan, Xia. et al. "Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews ( Tupaia belangeri)" Comparative medicine, 2023.
During the reproductive season, tree shrews exhibit distinct reproductive cycles, with sexually mature male tree shrews producing limited or no sperm with low motility. The underlying mechanism for this phenomenon remains unknown. A team of researchers hypothesized that testis-specific serine/threonine kinases (TSSK) may play a crucial role in spermatogenesis or sperm function regulation in tree shrews, given their specific expression in the testis and male germ cells of mammals. However, the expression, distribution, subcellular localization, and dynamic changes of TSSK in tree shrew sperm are still unclear. In this study, it was found that during the reproductive season, the seminiferous tubules were notably larger and contained mature sperm and other germ cells, compared to the nonreproductive season. The mRNA expression of Tree Shrew TSSK was also evaluated.
Tan, Xia. et al. "Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews ( Tupaia belangeri)" Comparative medicine, 2023.
Pubmed: 37550055 DOI: 10.30802/AALAS-CM-23-000043

Anbazhagan, Rajakumar. et al. "miRNA Expression Profiles of Mouse Round Spermatids in GRTH/DDX25-Mediated Spermiogenesis: mRNA-miRNA Network Analysis." Cells, 2023.
The testis-specific DEAD-box family of RNA helicase GRTH/DDX25 is critical for spermatogenesis and male fertility. This family has two forms: a 56 kDa non-phosphorylated form and a 61 kDa phosphorylated form (pGRTH). Sterile GRTH-KO and GRTH Knock-In (KI) mice with a R242H mutation (lacking pGRTH) have a spermatogenic arrest at the RS development stage, causing spermatids to fail to elongate. Researchers performed mRNA-seq and miRNA-seq analyses on RS from wild-type, KI, and KO mice to identify key miRNAs and mRNAs during RS development and established a miRNA-mRNA network. miR146, miR122a, miR26a, miR27a, miR150, miR196a, and miR328 were among the increased levels of miRNAs that were found to be relevant to spermatogenesis. DE-miRNA and DE-mRNA target analyses showed that these miRNAs target genes involved in processes such as ubiquitination (CR, Hrd1, cullins, Nedd4l) and steps of spermiogenesis (par-1a, GAPDS, Rhox5, Pnpla5).
Anbazhagan, Rajakumar. et al. "miRNA Expression Profiles of Mouse Round Spermatids in GRTH/DDX25-Mediated Spermiogenesis: mRNA-miRNA Network Analysis." Cells, 2023.
Pubmed: 36899892 DOI: 10.3390/cells12050756