mProX™ Human STK26 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX394	Magic™ Human MST4 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;MEF

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research

Related Diseases

Cerebral Cavernous Malformations 3; Cerebrocostomandibular Syndrome

Gene ID

Human:51765

UniProt ID

Human:Q9P289

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

STK26, also known as mammalian sterile 20-like kinase 4 (MST4), is a serine/threonine protein kinase that plays a role in various biological processes, including cell migration, proliferation, invasion, autophagy signaling, and tumor progression. It has been implicated in different diseases, such as cerebral ischemia-reperfusion injury, hepatocellular carcinoma, mammary adenocarcinoma, and tumor progression. STK26 is associated with the treatment of cerebral ischemia-reperfusion by breviscapine therapy and is identified as one of the m(6)A methylation-related differentially expressed genes (DEGs). It also plays a critical role in promoting the progression of hepatocellular carcinoma and is a potential therapeutic target for liver cancer. In mammary adenocarcinoma, STK26 is differentially expressed in cells with erythropoietin receptor overexpression. Furthermore, STK26 functions as an oncogene and is involved in autophagy signaling and treatment resistance. In bighead carp, STK26 is one of the members of the STK gene family and is associated with immune regulation and defense against pathogens. Overall, the applications of STK26 in various diseases and biological processes suggest its potential as a target for therapeutic interventions.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Taylor Johnson (Verified Customer)

What are the biological processes involving STK26? Jul 03 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

STK26, part of the mammalian sterile 20-like (MST) family, is involved in a variety of biological processes, including cell apoptosis, immune regulation, and cancer. Jul 03 2022

chat Peyton Miller (Verified Customer)

How is STK26 expression correlated with gastric cancer? Nov 15 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

STK26 mRNA expression negatively correlates with certain gastric cancer markers, suggesting its potential role in gastric tumorigenesis. Nov 15 2020

Published Data

Fig.1 Silencing STK26 MST4) in MEF cells.

In MEFs, control shRNA transduction with a scrambled sequence was employed, resulting in the induction of apparent IκBα degradation and downstream kinase activation (IKKβ, Jnk, and Erk) along with a reduction in MST4 protein abundance upon acute LPS challenge. Nevertheless, MST4 knockdown induced by shRNA further facilitated LPS-mediated signal transduction, characterized by an intensified IκBα degradation and enhanced phosphorylation of downstream kinases (IKKβ and Erk).

Ref: Jiao, Shi, et al. "The kinase MST4 limits inflammatory responses through direct phosphorylation of the adaptor TRAF6." Nature immunology 16.3 (2015): 246-257.

Pubmed: 25642822

DOI: 10.1038/ni.3097

Research Highlights

Wan, Cheng. et al. "Identification of m6A methylation-related genes in cerebral ischaemia‒reperfusion of Breviscapus therapy based on bioinformatics methods." BMC medical genomics, 2023.
In this study, researchers investigated the effects of breviscapine on cerebral ischaemia‒reperfusion (I/R)-induced neuronal damage using transcriptome sequencing in mice. They identified 2250 differentially expressed genes (DEGs) between the OGD/R and control groups and 757 DEGs between the Therapy and OGD/R groups. Moreover, seven m6A-related DEGs were pinpointed, and four key genes (Tfcp2l1, Kcns3, Olfml2a, and Arl4d) were identified, highlighting their involvement in DNA repair, e2f targets, and the g2m checkpoint. Tfcp2l1 was found to be crucial in human embryonic stem cell pluripotency. The study also revealed a circRNA-miRNA‒mRNA network regulating Tfcp2l1. These findings offer valuable insights into breviscapine's potential for OGD/R treatment, with implications for future clinical trials.
Wan, Cheng. et al. "Identification of m6A methylation-related genes in cerebral ischaemia‒reperfusion of Breviscapus therapy based on bioinformatics methods." BMC medical genomics, 2023.
Pubmed: 37670341 DOI: 10.1186/s12920-023-01651-3

Caputo, Mara. et al. "STE20-type kinases MST3 and MST4 promote the progression of hepatocellular carcinoma: Evidence from human cell culture and expression profiling of liver biopsies." FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2023.
Hepatocellular carcinoma (HCC) is a highly deadly and rapidly spreading form of cancer. Recently, the role of nonalcoholic steatohepatitis (NASH) in promoting HCC has been recognized. This study focuses on the contribution of two protein molecules, MST3 and MST4, in the development of HCC. Through analysis of various datasets and patient samples, the researchers found a positive correlation between the expression of MST3 and MST4 and the incidence and severity of HCC. Their experiments also showed that inhibiting these proteins significantly reduced the growth and invasiveness of HCC cells. Further investigations revealed that MST3 and MST4 interact with GOLGA2 and the STRIPAK complex to activate the STAT3 signaling pathway, which plays a crucial role in HCC progression. These findings suggest that targeting MST3 and MST4 may provide a promising therapeutic approach for HCC treatment.
Caputo, Mara. et al. "STE20-type kinases MST3 and MST4 promote the progression of hepatocellular carcinoma: Evidence from human cell culture and expression profiling of liver biopsies." FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2023.
Pubmed: 37490000 DOI: 10.1096/fj.202300397RR