mProX™ Human PASK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX421	Magic™ Human PASK in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;L6

Target Classification

Kinase Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Niemann-Pick Disease, Type C1

Gene ID

Human:27347

UniProt ID

Human:Q9UEW8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PASK (Per-Arnt-Sim Kinase) has various applications in different fields. In the field of biomedicine, PASK is involved in the identification of molecular subgroups of CD8+ T cell-associated genes in ovarian cancer and predicting the response to immunotherapy. It has been found that a gene signature including PASK can be used for accurate prognosis and prediction of immunotherapeutic response in ovarian cancer patients. In the context of ischemic stroke, PASK is involved in regulating microglia polarization, and the compound Jasminoidin has been found to reduce stroke injury by modulating PASK-EEF1A1 axis in microglia. PASK is also implicated in the nuclear translocation of proteins, specifically in the case of PAS domain-containing proteins, which play important roles in cellular signaling. Additionally, PASK has been found to modulate the modification of histidine repeat proteins via inorganic polyphosphate, suggesting a potential regulatory role for PASK in protein function. Overall, PASK has diverse applications in different areas of research, including cancer, stroke, cellular signaling, and protein regulation.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Peyton Miller (Verified Customer)

What is the role of PASK in glycogen storage and utilization in the liver? Aug 18 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

PASK is crucial for the regulation of glycogen storage and utilization in the liver, especially during nutritional changes, as it is involved in GLP-1 dependent processes. Aug 18 2020

chat Taylor Brown (Verified Customer)

How does PASK affect oxidative stress in the liver? Jun 24 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

Inhibition of PASK, particularly under fasting conditions, could potentially ameliorate disorders associated with excess oxidative stress in the liver. Jun 24 2020

Published Data

Fig.1 Knockdown of PASK in L6 cells.

In the absence of doxycycline, PASK shRNA was expressed in L6 clones, and mRNA levels were measured for both scrambled and PASK shRNA-expressing groups. The mean ± SD of triplicates per group was calculated for data representation.

Ref: Hao, Huai-Xiang, et al. "PAS kinase is required for normal cellular energy balance." Proceedings of the National Academy of Sciences 104.39 (2007): 15466-15471.

Pubmed: 17878307

DOI: 10.1073/pnas.0705407104

Research Highlights

Zhu, Yunshu. et al. "Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response." Biomedicines, 2023.
The impact of infiltrating CD8+ T lymphocytes in the tumor microenvironment on the survival of ovarian cancer (OC) patients is still not fully understood. Currently, there is limited knowledge about the specific mechanism by which these cells affect patient survival. Further research is needed to clarify the role of infiltrating CD8+ T lymphocytes and their potential as a prognostic biomarker for OC. This information is critical for developing targeted therapies for OC patients with infiltrating CD8+ T lymphocytes present in their tumor microenvironment.
Zhu, Yunshu. et al. "Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response." Biomedicines, 2023.
Pubmed: 37760841 DOI: 10.3390/biomedicines11092399

Wu, Jinhan. et al. "Jasminoidin reduces ischemic stroke injury by regulating microglia polarization via PASK-EEF1A1 axis." Chemical biology & drug design, 2023.
In this study, the researchers aimed to investigate the molecular mechanism of Jasminoidin (JAS) in alleviating injury caused by ischemic stroke (IS). It is known that microglia polarization plays a crucial role in the progression of IS. To assess the link between JAS and microglia polarization, IS modeling was performed on mice by middle cerebral artery occlusion (MCAO), followed by treatment with JAS. Additionally, oxygen-glucose deprivation/reoxygenation (OGD/R) was used to induce microglia modeling in vitro, and JAS was administered to determine its effects. The results showed that JAS treatment reduced infarct volume, brain water content, and histological changes in the brain. It also reversed changes in the expression of various genes involved in microglia polarization. The findings suggest that JAS exerts its beneficial effects on IS by regulating microglia polarization via the PASK-EEF1A1 axis.
Wu, Jinhan. et al. "Jasminoidin reduces ischemic stroke injury by regulating microglia polarization via PASK-EEF1A1 axis." Chemical biology & drug design, 2023.
Pubmed: 37743322 DOI: 10.1111/cbdd.14354