mProX™ Human GPR101 Stable Cell Line

Product Information

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

Ishikawa;CHO-K1;HEK293

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

CNS Research;Metabolic Research

Related Diseases

Pituitary Adenoma 2, Growth Hormone-Secreting;Acromegaly

Gene ID

Human:83550

UniProt ID

Human:Q96P66

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

GPR101, a G-protein coupled receptor, has been identified as a significant player in the field of scientific research. Recent studies have highlighted its potential role in the regulation of growth hormone secretion, with mutations in the GPR101 gene being linked to X-linked acrogigantism (X-LAG), a condition characterized by an overproduction of growth hormone. This discovery has opened new avenues for understanding the molecular mechanisms underlying growth hormone-related disorders and offers potential therapeutic targets for conditions like X-LAG. As research progresses, the broader implications of GPR101 in endocrinology and its potential applications in therapeutic interventions are anticipated to be unveiled.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human GPR101 Stable Cell Line (S01YF-1023-PY314). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Nancy (Verified Customer)

What is the significance of GPR101 in acrogigantism and acromegaly? Aug 17 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

Mutations in GPR101 have been identified as a potential cause of X-linked acrogigantism and acromegaly. GPR101 is predominantly expressed in the hypothalamus and pituitary, suggesting its importance in regulating functions like energy balance and reproduction. Aug 17 2023

chat Matthew (Verified Customer)

How does GPR101 relate to gigantism? Jul 04 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

X-linked acrogigantism is associated with GPR101 mutations. While the physiological function and endogenous ligand of GPR101 remain unknown, its high expression in the arcuate nucleus and increased circulating GHRH levels in some patients suggest a role in hypothalamic GHRH secretion. Jul 04 2021

Published Data

Fig.1 Knockdown of GPR101 in Ishikawa cells.

The evaluation of GPR101 expression suppression through antisense oligonucleotide was conducted, where Ishikawa cells exposed to antisense oligonucleotide directed against GPR101 demonstrated a significant reduction in GPR101 mRNA levels, quantified via qPCR analysis. In contrast, cells subjected to the sense strand or transfection reagent (OF; oligofectamine) alone exhibited no discernible alterations in GPR101 expression levels. The results are presented as mean ± SEM (n = 4), with significance denoted by *, indicating P < .05 compared to the vehicle control.

Ref: Cho-Clark, Madelaine, et al. "GnRH-(1-5) transactivates EGFR in Ishikawa human endometrial cells via an orphan G protein-coupled receptor." Molecular endocrinology 28.1 (2014): 80-98.

Pubmed: 24264576

DOI: 10.1210/me.2013-1203

Research Highlights

Constantine A Stratakis "Genes and environment: An old pair in a new era." Maturitas, 2023
In this review, the authors present a synthesis of the Lamarckian and Darwinian theories in regards to how environmental pressures can impact the inheritance of genetic changes through epigenetics. While Lamarck's concept of inheritance of acquired traits may play a role, the basis of evolution and sustained inheritance is still rooted in Darwin's theory of natural selection. This understanding is crucial in comprehending how our health and that of our descendants responds to rapid shifts in the environment. The authors also provide additional examples of how environmental cues can lead to changes in disease frequency and expression.
Constantine A Stratakis "Genes and environment: An old pair in a new era." Maturitas, 2023
Pubmed: 37806009 DOI: 10.1016/j.maturitas.2023.107851

Constantine A Stratakis "O tempora, o mores: The Age We Live In, Machine Learning, Hypertension, and Primary Aldosteronism." JACC. Asia, 2023
The study aims to investigate the effect of social media usage on student's academic performance. Data will be collected through a survey distributed to undergraduate students. Results will be analyzed using statistical methods. The study hypothesizes that high usage of social media will have a negative impact on academic performance. This research is important in understanding the relationship between social media and academic success, and can have implications for educational institutions and policies.
Constantine A Stratakis "O tempora, o mores: The Age We Live In, Machine Learning, Hypertension, and Primary Aldosteronism." JACC. Asia, 2023
Pubmed: 37614549 DOI: 10.1016/j.jacasi.2023.06.005