mProX™ Human FCGR2C Stable Cell Line

Typiak, Marlena. et al. "Comparative Analysis of FCGR Gene Polymorphism in Pulmonary Sarcoidosis and Tuberculosis" Cells, 2023.
The clinical outcome of sarcoidosis (SA) is very similar to tuberculosis (TB); however, they are treated differently and should not be confused. In search for their biomarkers, we have previously revealed changes in the phagocytic activity of monocytes in sarcoidosis and tuberculosis. On these monocytes we found a higher expression of receptors for the Fc fragment of immunoglobulin G (FcγR) in SA and TB patients vs. healthy controls. FcγRs are responsible for the binding of immune complexes (ICs) to initiate an (auto)immune response and for ICs clearance. Surprisingly, our SA patients had a high blood level of ICs, despite the abundant presence of FcγRs. It pointed to FcγR disfunction, presumably caused by the polymorphism of their (FCGR) genes. Therefore, we present here an analysis of the occurrence of FCGR2A, FCGR2B, FCGR2C, FCGR3A and FCGR3B variants in Caucasian SA and TB patients, and healthy individuals with the use of polymerase chain reaction (PCR) and real-time PCR. The presented data point to a possibility of supporting the differential diagnosis of SA and TB by analyzing FCGR2C, FCGR3A and FCGR3B polymorphism, while for severe stages of SA also by studying FCGR2A variants. Additionally, the genotyping of FCGR2A and FCGR3B might serve as a marker of SA progression.
Typiak, Marlena. et al. "Comparative Analysis of FCGR Gene Polymorphism in Pulmonary Sarcoidosis and Tuberculosis" Cells, 2023.
Pubmed: 37174624   DOI: 10.3390/cells12091221

Kløve-Mogensen, Kirstine. et al. "Genetic variations in low-to-medium-affinity Fcγ receptors and autoimmune neutropenia in early childhood in a Danish cohort" International journal of immunogenetics, 2023.
Autoimmune neutropenia (AIN) during early childhood results from autoantibodies targeting neutrophil membrane antigens and represents a common cause of childhood neutropenia. This study delves into genetic variations within the FCGR locus and FCGR3B copy number variations. Among 130 AIN patients, 64 with anti-HNA-1a antibodies and 66 with broad-reacting anti-FcγRIIIb antibodies, genotyping via multiplex ligation probe assay and real-time q-PCR compared them with healthy controls. The research reveals associations specific to antibody types, notably in the anti-HNA-1a-positive group and the HNA-1 genotype. The presence of FCGR3B copy, HNA genotypes, FCGR2A 166H, FCGR2B 232I, and FCGR2B 2B.4 copies increased AIN risk, while HNA-1bb, FCGR2A 166R, FCGR2B 232T, and one FCGR2B promoter 2B.4 copy decreased risk. These findings underscore a robust FCGR locus-AIN association in the Danish cohort and suggest potential disease heterogeneity.
Kløve-Mogensen, Kirstine. et al. "Genetic variations in low-to-medium-affinity Fcγ receptors and autoimmune neutropenia in early childhood in a Danish cohort" International journal of immunogenetics, 2023.
Pubmed: 36754570   DOI: 10.1111/iji.12614