mProX™ Human CDC42BPA Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1228	Magic™ Human MRCKα(CDC42BPA) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;HCT116;RKO

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Myotonic Dystrophy

Gene ID

Human:8476

UniProt ID

Human:Q5VT25

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

CDC42BPA is a protein that plays a role in various biological processes. In the context of neuronal function and plasticity, CDC42BPA is involved in the transcription of genes related to plasticity and cognitive resilience. It is activated by reduced glucose availability and can induce ER stress, leading to the transcription of genes involved in plasticity and cognitive function. In the acrosome reaction of Eriocheir sinensis spermatozoa, CDC42BPA is one of the hub proteins identified in the PPI network and is involved in the regulation of cytoskeleton organization and small GTPase-mediated signal transduction. In the study on chronic obstructive pulmonary disease (COPD), CDC42BPA is one of the hub genes identified in COPD network clusters and is connected to dysregulations of protein degradation, inflammatory cytokine production, airway remodeling, and immune cell activity. It is also identified as a prognostic marker in lung cancer. Furthermore, in the study on acute lung injury, gene transfer of MRCKŒ±, which interacts with CDC42BPA, can rescue alveolar capillary barrier function and attenuate lung injury. Overall, CDC42BPA is implicated in various biological processes, including neuronal plasticity, spermatozoa acrosome reaction, COPD, and lung injury.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Morgan Garcia (Verified Customer)

CDC42BPA
Q: Is CDC42BPA implicated in any specific diseases? Apr 23 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

CDC42BPA, also known as MRCKα, has been identified as a novel regulator and candidate therapeutic target in multiple myeloma, suggesting its significance in this type of bone marrow cancer. Apr 23 2020

chat Cameron Jones (Verified Customer)

How does CDC42BPA contribute to cancer progression? Dec 16 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

While specific mechanisms are still under investigation, CDC42BPA's role in multiple myeloma indicates a potential impact on tumor clonality and early progression of the disease. Dec 16 2020

Published Data

Fig.1 knockdown of CDC42BPA significantly reduced invasive potential of I8 cells in a dose-dependent manner.

Ref: Hu, Hui-Fang, et al. "Comparative proteomics analysis identifies Cdc42-Cdc42BPA signaling as prognostic biomarker and therapeutic target for colon cancer invasion." Journal of proteome research 17.1 (2018): 265-275.

Pubmed: 29072916

DOI: 10.1021/acs.jproteome.7b00550

Research Highlights

Kumar, Amit. et al. "2-Deoxyglucose drives plasticity via an adaptive ER stress-ATF4 pathway and elicits stroke recovery and Alzheimer's resilience." Neuron, 2023.
Intermittent fasting (IF) has been found to have beneficial effects on cognitive aging, Alzheimer's disease (AD), and stroke. It involves restricting a number of nutrients, including glucose. The glucose analog, 2-deoxyglucose (2-DG), can simulate glucose restriction. Recent studies have shown that 2-DG can increase the expression of Bdnf, a pro-plasticity factor, in the brain without the body going into ketosis. This results in improved memory in an AD model (5xFAD) and enhanced recovery in an ischemic stroke model. Further research has revealed that 2-DG induces the production of Bdnf by reducing N-linked glycosylation, leading to endoplasmic reticulum stress and activation of ATF4 at the Bdnf gene. This process also affects the regulation of other plasticity and regeneration-related genes, such as Creb5, Cdc42bpa, Ppp3cc, and Atf3. These findings highlight the importance of N-linked glycosylation in perceiving reduced glucose levels and demonstrate that ER stress, caused by 2-DG without ketosis, can trigger the transcription of genes involved in plasticity, cognitive resilience, and proteostasis.
Kumar, Amit. et al. "2-Deoxyglucose drives plasticity via an adaptive ER stress-ATF4 pathway and elicits stroke recovery and Alzheimer's resilience." Neuron, 2023.
Pubmed: 37453419 DOI: 10.1016/j.neuron.2023.06.013

Tang, Yulian. et al. "Role of cytoskeleton-related proteins in the acrosome reaction of Eriocheir sinensis spermatozoa." BMC genomic data, 2023.
Cytoskeletal proteins play a vital role in maintaining cell shape and remodeling the cytoskeleton. The study focused on understanding the mechanism of spermatozoal metamorphosis during the acrosome reaction in Eriocheir sinensis (Chinese mitten crab) spermatozoa. They induced the acrosome reaction in vitro using calcium ionophore A23187 and collected acrosome-reacting and fresh spermatozoa separately. Through transcriptome sequencing and bioinformatics analysis, they identified numerous differentially expressed cytoskeleton-related protein genes, miRNAs, and lncRNAs. In the protein-protein interaction networks, several hub proteins, including RAC1, BCAR1, RDX, NCKAP1, EPS8, CDC42BPA, LIMK1, ELMO2, GNAI1, and OCRL, were implicated in regulating cytoskeleton organization and other processes. In the miRNA-mRNA regulatory network, core miRNAs such as miR-9 and miR-31 targeted cytoskeleton-related protein genes, shedding light on the acrosome reaction mechanism in E. sinensis spermatozoa with unique morphology.
Tang, Yulian. et al. "Role of cytoskeleton-related proteins in the acrosome reaction of Eriocheir sinensis spermatozoa." BMC genomic data, 2023.
Pubmed: 36782118 DOI: 10.1186/s12863-023-01112-x